{"medium":{"id":1012,"name":"PEREDIBACTER (DN) MEDIUM","complex_medium":"yes","min_pH":7.2,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1012.pdf"},"solutions":[{"id":2063,"name":"Main sol. 1012","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nutrient broth","compound_id":269,"attribute":"BD Difco","amount":0.8,"unit":"g","g_l":0.8,"optional":"no"},{"recipe No.":2,"compound":"Casamino acids","compound_id":101,"attribute":"BD Bacto","amount":0.5,"unit":"g","g_l":0.5,"optional":"no"},{"recipe No.":3,"compound":"Yeast extract","compound_id":16,"attribute":"OXOID","amount":0.1,"unit":"g","g_l":0.1,"optional":"no"},{"recipe No.":4,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":2.04061,"optional":"no"},{"recipe No.":5,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.6,"unit":"g","g_l":0.6,"mmol_l":2.95126,"optional":"no"},{"recipe No.":6,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except calcium and magnesium chloride and adjust pH of the medium to 7.2 with NaOH. After autoclaving add calcium and magnesium from sterile stock solutions."},{"step":"Before inoculation prepare a suspension of prey cells in 30-40 ml medium using a 250 ml flask. Usually the resuspension of two overnight cultures of the prey strain on agar slants of DSMZ medium 54 will yield a final concentration of 10<sup>8</sup> to 10<sup>9</sup> cells per ml culture broth."}],"equipment":{"4742":"Autoclave"},"main solution":"yes"}]}