{"medium":{"id":"1012a","name":"BDELLOVIBRIO BACTERIOVORUS MEDIUM","complex_medium":"yes","min_pH":7.2,"max_pH":7.2,"source":"DSMZ","description":""},"solutions":[{"id":6336,"name":"Main sol. 1012a","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nutrient broth","compound_id":269,"attribute":"BD Difco","amount":0.8,"unit":"g","g_l":0.8,"optional":"no"},{"recipe No.":2,"compound":"Casamino acids","compound_id":101,"attribute":"BD Bacto","amount":0.5,"unit":"g","g_l":0.5,"optional":"no"},{"recipe No.":3,"compound":"Yeast extract","compound_id":16,"attribute":"OXOID","amount":0.1,"unit":"g","g_l":0.1,"optional":"no"},{"recipe No.":4,"compound":"Agar","compound_id":3,"attribute":"BD Bacto","amount":12,"unit":"g","g_l":12,"optional":"no"},{"recipe No.":5,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.6,"unit":"g","g_l":0.6,"mmol_l":2.95126,"optional":"no"},{"recipe No.":6,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":2.04061,"optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Preparation of double-layered agar plates:

\r\n\r\nBottom layer:\r\nDissolve ingredients and adjust the pH of the medium to 7.2 with NaOH. Then bring the medium to a boil to dissolve the agar and autoclave (121\u00b0C for 15 min). Dry plates at room temperature for 2 - 3 days.

\r\n\r\nTop layer:\r\nGrow prey bacterium on agar slants using DSMZ medium 54 or medium 1. Prepare 5 ml aliquots of 1 % Bacto agar in 25 mM HEPES buffer (6.0 g/l HEPES, 0.6 g/l MgCl2 x 6 H2O, 0.3 g/l CaCl2 x 2 H2O, pH 7.2 ) and autoclave in test tubes. After cooling to 45\u00b0C in a water bath add 0.5 ml cell suspension of the host organism, mix, and pour the top layer. For the top layer of one plate use one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.

"},{"step":"Inoculate the top layer of the double-layer plates the same day when prepared."}],"equipment":{"5201":"Autoclave","5202":"Petri dishes"},"main solution":"yes"}]}