{"medium":{"id":1416,"name":"MST-MEDIUM, AUTOTROPHIC GROWTH","complex_medium":"no","min_pH":7.5,"max_pH":8,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1416.pdf"},"solutions":[{"id":2886,"name":"Main sol. 1416","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2S2O3 x 5 H2O","compound_id":65,"amount":5,"unit":"g","g_l":5,"mmol_l":20.1462,"optional":"no"},{"recipe No.":2,"compound":"NH4Cl","compound_id":42,"amount":1,"unit":"g","g_l":1,"mmol_l":18.6951,"optional":"no"},{"recipe No.":3,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.02859,"optional":"no"},{"recipe No.":4,"compound":"K2HPO4","compound_id":10,"amount":4,"unit":"g","g_l":4,"mmol_l":22.9653,"optional":"no"},{"recipe No.":5,"compound":"KH2PO4","compound_id":11,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":11.0225,"optional":"no"},{"recipe No.":6,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Prepare separate solutions of sodium thiosulfate and magnesium sulfate. Adjust solutions to pH 7.5 -8.0 and sterilize by autoclaving. Add sterile trace element solution after autoclving and combining the solutions."}],"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"}]}