{"medium":{"id":"1630c","name":"modified Nitrosospaera medium","complex_medium":"no","min_pH":7.5,"max_pH":7.5,"source":"DSMZ","description":"based on M. 1630, but modified after Melcher&Schleper during deposition in 2021-2022"},"solutions":[{"id":6278,"name":"Main sol. 1630c","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1,"mmol_l":17.1116,"optional":"no"},{"recipe No.":2,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.4,"unit":"g","g_l":0.4,"mmol_l":1.96751,"optional":"no"},{"recipe No.":3,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.680203,"optional":"no"},{"recipe No.":4,"compound":"KH2PO4","compound_id":11,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":1.46967,"optional":"no"},{"recipe No.":5,"compound":"KCl","compound_id":61,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":6.70691,"optional":"no"},{"recipe No.":6,"compound":"Na2SO4","compound_id":110,"amount":0.05,"unit":"g","g_l":0.05,"mmol_l":0.352014,"optional":"no"},{"recipe No.":7,"solution":"Trace element solution (Nitrososphaera)","solution_id":6280,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":8,"solution":"FeNaEDTA solution (7.5 mM)","solution_id":6281,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":9,"solution":"NaHCO3 buffer (1M)","solution_id":6282,"amount":2,"unit":"ml","optional":"no"},{"recipe No.":10,"solution":"HEPES buffer solution","solution_id":3383,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":11,"solution":"NH4Cl (1 M)","solution_id":6283,"amount":2,"unit":"ml","optional":"no"},{"recipe No.":12,"solution":"Sodiumpyruvate (1 M)","solution_id":6284,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":13,"compound":"Double distilled water","compound_id":1021,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Incubate all used glass ware and magnetic stirring rods in 0.1 M HCl and rinse 3x with double distilled water to remove residual detergents (always check visual for soap bubbles)."},{"step":"Autoclave the Basic-FWM and cool to room temperature. This medium should be free of any precipitations!"},{"step":"Aseptically, add per L of BASIC-FWM the following solutions from sterile stocks (do not store complemented FWM):\r\n- Trace element solution (Nitrososphaera)\r\n- FeNaEDTA solution\r\n- NaHCO3 solution\r\n- HEPES buffer solution\r\n- NH4Cl solution\r\n- Sodiumpyruvate solution"},{"step":"Adjust pH to 7.5."}],"equipment":{"5162":"Autoclave","5161":"Filter"},"main solution":"yes"},{"id":6280,"name":"Trace element solution (Nitrososphaera)","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"12.5M","amount":8,"unit":"mL","g_l":3.646,"mmol_l":100,"optional":"no"},{"recipe No.":2,"compound":"H3BO3","compound_id":37,"amount":30,"unit":"mg","g_l":0.03,"mmol_l":0.485123,"optional":"no"},{"recipe No.":3,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":4,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":5,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":6,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":7,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":144,"unit":"mg","g_l":0.144,"mmol_l":0.500763,"optional":"no"},{"recipe No.":8,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":9,"compound":"Double distilled water","compound_id":1021,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"This solution contains no iron! Sterilise by autoclaving or filtration (0.2 \u00b5m) and store in the dark at 4 \u00b0C."}],"equipment":{"5165":"Filter"},"main solution":"no"},{"id":3383,"name":"HEPES buffer solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"attribute":"pellets","amount":24,"unit":"g","g_l":24,"mmol_l":600.045,"optional":"no"},{"recipe No.":2,"compound":"HEPES","compound_id":371,"attribute":"free acid","amount":238.4,"unit":"g","g_l":238.4,"mmol_l":1000.42,"optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume."}],"main solution":"no"},{"id":6281,"name":"FeNaEDTA solution (7.5 mM)","volume":1000,"recipe":[{"recipe No.":2,"compound":"FeNa-EDTA","compound_id":1230,"amount":2753,"unit":"mg","g_l":2.753,"optional":"no"},{"recipe No.":3,"compound":"Double distilled water","compound_id":1021,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Sterilise by filtration (0.2 \u00b5m) and store in the dark at 4 \u00b0C."}],"equipment":{"5164":"Filter"},"main solution":"no"},{"id":6282,"name":"NaHCO3 buffer (1M)","volume":50,"recipe":[{"recipe No.":2,"compound":"NaHCO3","compound_id":55,"amount":4.2,"unit":"g","g_l":84,"mmol_l":999.917,"optional":"no"},{"recipe No.":3,"compound":"Double distilled water","compound_id":1021,"amount":50,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 \u00b5m) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 \u00b0C. Avoid high headspace to liquid ratios to ensure stable concentration."}],"equipment":{"5166":"Filter"},"main solution":"no"},{"id":6283,"name":"NH4Cl (1 M)","volume":50,"recipe":[{"recipe No.":2,"compound":"NH4Cl","compound_id":42,"amount":2.67,"unit":"g","g_l":53.4,"mmol_l":998.317,"optional":"no"},{"recipe No.":3,"compound":"Double distilled water","compound_id":1021,"amount":50,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water."},{"step":"Filtrate (0.2 \u00b5m)."}],"equipment":{"5167":"Filter"},"main solution":"no"},{"id":6284,"name":"Sodiumpyruvate (1 M)","volume":50,"recipe":[{"recipe No.":2,"compound":"Sodium pyruvate","compound_id":438,"amount":5.5,"unit":"g","g_l":110,"mmol_l":999.636,"optional":"no"},{"recipe No.":3,"compound":"Double distilled water","compound_id":1021,"amount":50,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 5.5 g in 50 mL and filter it through 0.2 \u00b5m sterile filter in a sterile falcon tube. Store at 4 \u00b0C"}],"equipment":{"5168":"Filter"},"main solution":"no"}]}