{"medium":{"id":1646,"name":"GEMMATIMONAS MEDIUM","complex_medium":"yes","min_pH":7,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1646.pdf"},"solutions":[{"id":3407,"name":"Main sol. 1646","volume":1000,"recipe":[{"recipe No.":1,"compound":"Yeast extract","compound_id":16,"amount":0.25,"unit":"g","g_l":0.25,"optional":"no"},{"recipe No.":2,"compound":"Peptone","compound_id":1,"attribute":"Difco","amount":0.5,"unit":"g","g_l":0.5,"optional":"no"},{"recipe No.":3,"compound":"Glucose","compound_id":5,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.555062,"optional":"no"},{"recipe No.":4,"compound":"HEPES","compound_id":371,"amount":1.95,"unit":"g","g_l":1.95,"mmol_l":8.18296,"optional":"no"},{"recipe No.":5,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.37,"unit":"g","g_l":0.37,"mmol_l":1.50116,"optional":"no"},{"recipe No.":6,"compound":"CaSO4 x 2 H2O","compound_id":190,"amount":0.43,"unit":"g","g_l":0.43,"mmol_l":2.4975,"optional":"no"},{"recipe No.":7,"compound":"Agar","compound_id":3,"amount":14,"unit":"g","g_l":14,"mmol_l":41.6258,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":21,"solution":"Vitamin solution","solution_id":926,"amount":5,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.0 prior to the addition of agar."},{"step":"After autoclaving and cooling to about 55\u00b0C, add 5 ml of vitamin solution."}],"equipment":{"794":"Filter"},"main solution":"yes"},{"id":926,"name":"Vitamin solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Vitamin B12","compound_id":18,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.0368895,"optional":"no"},{"recipe No.":2,"compound":"Pantothenic acid","compound_id":135,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.228071,"optional":"no"},{"recipe No.":3,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":4,"compound":"Pyridoxamine hydrochloride","compound_id":1513,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.048863,"optional":"no"},{"recipe No.":5,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":6,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":7,"compound":"Nicotinic acid","compound_id":139,"amount":25,"unit":"mg","g_l":0.025,"mmol_l":0.20307,"optional":"no"},{"recipe No.":8,"compound":"Nicotine amide","compound_id":140,"amount":25,"unit":"mg","g_l":0.025,"mmol_l":0.204717,"optional":"no"},{"recipe No.":9,"compound":"alpha-lipoic acid","compound_id":141,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":10,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":11,"compound":"Thiamine-HCl x 2 H2O","compound_id":77,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.13394,"optional":"no"},{"recipe No.":12,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Stir for some hours, filter sterilize the solution."}],"equipment":{"2811":"Filter"},"Sol. No.":11,"main solution":"no"}]}