{"medium":{"id":2,"name":"BACILLUS PASTEURII MEDIUM","complex_medium":"yes","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium2.pdf"},"solutions":[{"id":4,"name":"Main sol. 2","volume":1000,"recipe":[{"recipe No.":1,"solution":"Main sol. 1","solution_id":1,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Urea","compound_id":339,"amount":20,"unit":"g/l","g_l":20,"mmol_l":333.023,"optional":"no"}],"steps":[{"step":"To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea."},{"step":"For sporulation enhancement add 10 mg/l MnSO4 x H2O."}],"equipment":{"3962":"Autoclave"},"main solution":"yes"},{"id":1,"name":"Main sol. 1","volume":1000,"recipe":[{"recipe No.":1,"compound":"Peptone","compound_id":1,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":2,"compound":"Meat extract","compound_id":2,"amount":3,"unit":"g","g_l":3,"optional":"no"},{"recipe No.":3,"compound":"Agar","compound_id":3,"condition":"for solid medium","amount":15,"unit":"g","g_l":15,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.0."},{"step":"For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation."}],"main solution":"no"}]}