{"medium":{"id":26,"name":"ACID RHODOSPIRILLACEAE MEDIUM","complex_medium":"yes","min_pH":5.7,"max_pH":5.7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium26.pdf"},"solutions":[{"id":5551,"name":"Main sol. 26","volume":1000,"recipe":[{"recipe No.":1,"compound":"Yeast extract","compound_id":16,"amount":0.2,"unit":"g","g_l":0.2,"optional":"no"},{"recipe No.":2,"compound":"Disodium succinate","compound_id":1189,"amount":1,"unit":"g","g_l":1,"mmol_l":6.17093,"optional":"no"},{"recipe No.":3,"compound":"Ammonium acetate","compound_id":58,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":6.48677,"optional":"no"},{"recipe No.":4,"compound":"Fe(III) citrate","compound_id":45,"attribute":"0.1% in H2O","amount":5,"unit":"ml","g_l":0.005,"mmol_l":0.0204128,"optional":"no"},{"recipe No.":5,"compound":"KH2PO4","compound_id":11,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":3.67417,"optional":"no"},{"recipe No.":6,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.4,"unit":"g","g_l":0.4,"mmol_l":1.62288,"optional":"no"},{"recipe No.":7,"compound":"NaCl","compound_id":43,"amount":0.4,"unit":"g","g_l":0.4,"mmol_l":6.84463,"optional":"no"},{"recipe No.":8,"compound":"NH4Cl","compound_id":42,"amount":0.4,"unit":"g","g_l":0.4,"mmol_l":7.47803,"optional":"no"},{"recipe No.":9,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.05,"unit":"g","g_l":0.05,"mmol_l":0.340101,"optional":"no"},{"recipe No.":10,"compound":"Vitamin B12","compound_id":18,"attribute":"10 mg in 100 ml H2O","amount":0.4,"unit":"ml","optional":"no"},{"recipe No.":11,"solution":"Trace element solution SL-6","solution_id":25,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":12,"compound":"L-Cysteine HCl","compound_id":1157,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":1.90331,"optional":"no"},{"recipe No.":13,"compound":"Resazurin","compound_id":25,"attribute":"0,1%","amount":0.5,"unit":"ml","g_l":0.005,"mmol_l":0.021816,"optional":"no"},{"recipe No.":14,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 5.7."},{"step":"Boil the medium for a few minute."},{"step":"Sparge medium with 100% N2 gas mixture to make it anoxic."},{"step":"Fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas."},{"step":"Autoclave at 121 \u00b0C for 20 min."},{"step":"Sterile syringes are used to inoculate and remove samples."},{"step":"Incubate in the light using a tungsten lamp."}],"equipment":{"3880":"Tungsten lamp","3881":"Autoclave"},"main solution":"yes"},{"id":25,"name":"Trace element solution SL-6","volume":1000,"recipe":[{"recipe No.":1,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.347752,"optional":"no"},{"recipe No.":2,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.151588,"optional":"no"},{"recipe No.":3,"compound":"H3BO3","compound_id":37,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":4.85123,"optional":"no"},{"recipe No.":4,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":1.54037,"optional":"no"},{"recipe No.":5,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0586569,"optional":"no"},{"recipe No.":6,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.154322,"optional":"no"},{"recipe No.":7,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.123993,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":1,"main solution":"no"}]}