{"medium":{"id":582,"name":"15% MH MEDIUM","complex_medium":"yes","min_pH":7.5,"max_pH":7.5,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium582.pdf"},"solutions":[{"id":1247,"name":"Main sol. 582","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaCl","compound_id":43,"amount":121.5,"unit":"g","g_l":121.5,"mmol_l":2079.06,"optional":"no"},{"recipe No.":2,"compound":"MgCl2","compound_id":453,"amount":10.5,"unit":"g","g_l":10.5,"mmol_l":110.283,"optional":"no"},{"recipe No.":3,"compound":"MgSO4","compound_id":325,"amount":14.4,"unit":"g","g_l":14.4,"mmol_l":119.631,"optional":"no"},{"recipe No.":4,"compound":"CaCl2","compound_id":81,"amount":0.54,"unit":"g","g_l":0.54,"mmol_l":4.86574,"optional":"no"},{"recipe No.":5,"compound":"KCl","compound_id":61,"amount":3,"unit":"g","g_l":3,"mmol_l":40.2414,"optional":"no"},{"recipe No.":6,"compound":"NaHCO3","compound_id":55,"amount":0.09,"unit":"g","g_l":0.09,"mmol_l":1.07134,"optional":"no"},{"recipe No.":7,"compound":"NaBr","compound_id":353,"amount":0.039,"unit":"g","g_l":0.039,"mmol_l":0.379046,"optional":"no"},{"recipe No.":8,"compound":"Proteose peptone no. 3","compound_id":316,"attribute":"Difco","amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":9,"compound":"Yeast extract","compound_id":16,"attribute":"Difco","amount":10,"unit":"g","g_l":10,"optional":"no"},{"recipe No.":10,"compound":"Glucose","compound_id":5,"amount":1,"unit":"g","g_l":1,"mmol_l":5.55062,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Final pH 7.5"},{"step":"NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 20 g/l for solid media."}],"main solution":"yes"}]}