{"medium":{"id":816,"name":"SPOROTOMACULUM MEDIUM","complex_medium":"yes","min_pH":7.6,"max_pH":7.6,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium816.pdf"},"solutions":[{"id":1656,"name":"Main sol. 816","volume":1002,"recipe":[{"recipe No.":1,"solution":"Solution A","solution_id":1657,"amount":920,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"Solution B","solution_id":1658,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Solution C","solution_id":1659,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":4,"solution":"Solution D","solution_id":1660,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":5,"solution":"Solution E","solution_id":1661,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":6,"solution":"Solution F","solution_id":1662,"amount":5,"unit":"ml","optional":"no"},{"recipe No.":7,"solution":"Solution G","solution_id":1663,"amount":5,"unit":"ml","optional":"no"},{"recipe No.":8,"solution":"Solution H","solution_id":1664,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, C, F, G, and H are autoclaved separately under 100% N2 gas. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere and autoclaved. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be checked and adjusted to 7.6 with a sterile anoxic stock solution of Na2CO3 (5% w/v), if necessary."}],"equipment":{"4698":"Gassing station","4699":"Autoclave","4700":"Hungate tubes"},"main solution":"yes"},{"id":1657,"name":"Solution A","volume":920,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":0.2,"unit":"g","g_l":0.217391,"mmol_l":1.59747,"optional":"no"},{"recipe No.":2,"compound":"NH4Cl","compound_id":42,"amount":0.3,"unit":"g","g_l":0.326087,"mmol_l":6.09622,"optional":"no"},{"recipe No.":3,"compound":"KCl","compound_id":61,"amount":0.5,"unit":"g","g_l":0.543478,"mmol_l":7.29012,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1.08696,"mmol_l":18.5995,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.15,"unit":"g","g_l":0.163043,"mmol_l":1.10903,"optional":"no"},{"recipe No.":6,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.4,"unit":"g","g_l":0.434783,"mmol_l":2.13859,"optional":"no"},{"recipe No.":7,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0005,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":920,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1658,"name":"Solution B","volume":1,"recipe":[{"recipe No.":1,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1659,"name":"Solution C","volume":1,"recipe":[{"recipe No.":1,"solution":"Selenite-tungstate solution","solution_id":777,"amount":1,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1660,"name":"Solution D","volume":50,"recipe":[{"recipe No.":1,"compound":"Na2CO3","compound_id":32,"amount":2.5,"unit":"g","g_l":50,"mmol_l":471.752,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":50,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1661,"name":"Solution E","volume":10,"recipe":[{"recipe No.":1,"compound":"3-Hydroxybenzoic acid","compound_id":513,"amount":2.8,"unit":"g","g_l":280,"mmol_l":2027.22,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Neutralized with NaOH."}],"main solution":"no"},{"id":1662,"name":"Solution F","volume":5,"recipe":[{"recipe No.":1,"compound":"Yeast extract","compound_id":16,"amount":0.5,"unit":"g","g_l":100,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":5,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1663,"name":"Solution G","volume":5,"recipe":[{"recipe No.":1,"compound":"Trypticase","compound_id":74,"attribute":"BD BBL","amount":0.5,"unit":"g","g_l":100,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":5,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1664,"name":"Solution H","volume":10,"recipe":[{"recipe No.":1,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":30,"mmol_l":124.905,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":10,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":777,"name":"Selenite-tungstate solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":12.5009,"optional":"no"},{"recipe No.":2,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0114062,"optional":"no"},{"recipe No.":3,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0121267,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":3,"main solution":"no"}]}