{"medium":{"id":836,"name":"DESULFITOBACTERIUM PCP-1 MEDIUM","complex_medium":"yes","min_pH":7.5,"max_pH":7.5,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium836.pdf"},"solutions":[{"id":1709,"name":"Main sol. 836","volume":1003,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":0.27,"unit":"g","g_l":0.27,"mmol_l":1.98405,"optional":"no"},{"recipe No.":2,"compound":"K2HPO4","compound_id":10,"amount":0.35,"unit":"g","g_l":0.35,"mmol_l":2.00946,"optional":"no"},{"recipe No.":3,"compound":"NH4Cl","compound_id":42,"amount":0.53,"unit":"g","g_l":0.53,"mmol_l":9.90839,"optional":"no"},{"recipe No.":4,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.491877,"optional":"no"},{"recipe No.":5,"compound":"FeCl2 x 4 H2O","compound_id":260,"attribute":"0.1% w/v in 0.2 N HCl","amount":2,"unit":"ml","g_l":0.002,"mmol_l":0.0100598,"optional":"no"},{"recipe No.":6,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.07,"unit":"g","g_l":0.07,"mmol_l":0.476142,"optional":"no"},{"recipe No.":7,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":8,"compound":"Yeast extract","compound_id":16,"amount":1,"unit":"g","g_l":1,"optional":"no"},{"recipe No.":9,"compound":"Na-pyruvate","compound_id":113,"amount":6.05,"unit":"g","g_l":6.05,"mmol_l":54.978,"optional":"no"},{"recipe No.":10,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0005,"optional":"no"},{"recipe No.":11,"compound":"Na2CO3","compound_id":32,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":4.71751,"optional":"no"},{"recipe No.":12,"compound":"Na2SO3","compound_id":109,"amount":0.63,"unit":"g","g_l":0.63,"mmol_l":4.99802,"optional":"no"},{"recipe No.":13,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.05,"unit":"g","g_l":0.05,"mmol_l":0.208175,"optional":"no"},{"recipe No.":14,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary."}],"equipment":{"1082":"Hungate tubes","2113":"Filter","2851":"Autoclave","4440":"Gassing station"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"}]}