{"medium":{"id":858,"name":"ANAEROBIC CHOLESTEROL MEDIUM","complex_medium":"no","min_pH":6.8,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium858.pdf"},"solutions":[{"id":1751,"name":"Main sol. 858","volume":10,"recipe":[{"recipe No.":1,"solution":"Solution A","solution_id":1752,"amount":9,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"Solution B","solution_id":1753,"amount":0.5,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Solution C","solution_id":1754,"amount":0.025,"unit":"ml","optional":"no"},{"recipe No.":4,"solution":"Solution D","solution_id":1755,"amount":0.05,"unit":"ml","optional":"no"},{"recipe No.":5,"solution":"Solution E","solution_id":1756,"amount":0.025,"unit":"ml","optional":"no"},{"recipe No.":6,"solution":"Solution F","solution_id":1757,"amount":0.5,"unit":"ml","optional":"no"}],"steps":[{"step":"Prepare solution A, distribute approbiate amounts of cholesterol (e.g. 5 mg for 10 ml medium) and solution A in anaerobic tubes, and autoclave anaerobically under 80% N2 + 20% CO2. Solutions B and F are also autoclaved under these conditions. Solution C, D and E may be prepared aerobically. After cooling, add to 9 ml solution A: 500 \u00b5l sol. B, 500 \u00b5l sol. F, 25 \u00b5l (one drop) sol. C and E, each, and 50 \u00b5l sol. D under anaerobic conditions. Adjust pH to 7.0\u00b10.2."}],"main solution":"yes"},{"id":1752,"name":"Solution A","volume":200,"recipe":[{"recipe No.":1,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.1,"unit":"g","g_l":0.5,"mmol_l":2.02859,"optional":"no"},{"recipe No.":2,"compound":"NH4Cl","compound_id":42,"amount":0.1,"unit":"g","g_l":0.5,"mmol_l":9.34754,"optional":"no"},{"recipe No.":3,"compound":"CaCl2","compound_id":81,"amount":0.02,"unit":"g","g_l":0.1,"mmol_l":0.901063,"optional":"no"},{"recipe No.":4,"compound":"NaNO3","compound_id":187,"amount":0.16,"unit":"g","g_l":0.8,"mmol_l":9.41232,"optional":"no"},{"recipe No.":5,"compound":"Distilled water","compound_id":4,"amount":200,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1753,"name":"Solution B","volume":100,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":1,"unit":"g","g_l":10,"mmol_l":73.4835,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":100,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1754,"name":"Solution C","volume":1,"recipe":[{"recipe No.":1,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"}],"steps":[{"step":"Trace elements SL-10 (see medium 320)"}],"main solution":"no"},{"id":1755,"name":"Solution D","volume":1,"recipe":[{"recipe No.":1,"solution":"Vitamin solution","solution_id":926,"amount":1,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1756,"name":"Solution E","volume":1,"recipe":[{"recipe No.":1,"solution":"Selenite-tungstate solution","solution_id":777,"amount":1,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1757,"name":"Solution F","volume":100,"recipe":[{"recipe No.":1,"compound":"NaHCO3","compound_id":55,"amount":8.4,"unit":"g","g_l":84,"mmol_l":999.917,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":100,"unit":"ml","optional":"no"}],"equipment":{"1207":"Anaerobic workbench","3525":"Autoclave"},"main solution":"no"},{"id":926,"name":"Vitamin solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Vitamin B12","compound_id":18,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.0368895,"optional":"no"},{"recipe No.":2,"compound":"Pantothenic acid","compound_id":135,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.228071,"optional":"no"},{"recipe No.":3,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":4,"compound":"Pyridoxamine hydrochloride","compound_id":1513,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.048863,"optional":"no"},{"recipe No.":5,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":6,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":7,"compound":"Nicotinic acid","compound_id":139,"amount":25,"unit":"mg","g_l":0.025,"mmol_l":0.20307,"optional":"no"},{"recipe No.":8,"compound":"Nicotine amide","compound_id":140,"amount":25,"unit":"mg","g_l":0.025,"mmol_l":0.204717,"optional":"no"},{"recipe No.":9,"compound":"alpha-lipoic acid","compound_id":141,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":10,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":11,"compound":"Thiamine-HCl x 2 H2O","compound_id":77,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.13394,"optional":"no"},{"recipe No.":12,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Stir for some hours, filter sterilize the solution."}],"equipment":{"2811":"Filter"},"Sol. No.":11,"main solution":"no"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":777,"name":"Selenite-tungstate solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":12.5009,"optional":"no"},{"recipe No.":2,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0114062,"optional":"no"},{"recipe No.":3,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0121267,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":3,"main solution":"no"}]}