{"medium":{"id":"9a","name":"VY/2, REDUCED MEDIUM","complex_medium":"yes","min_pH":7.2,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium9a.pdf"},"solutions":[{"id":10,"name":"Main sol. 9a","volume":1000,"recipe":[{"recipe No.":1,"compound":"Baker's yeast","compound_id":17,"amount":2.5,"unit":"g","g_l":2.5,"optional":"no"},{"recipe No.":2,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":1.36,"unit":"g","g_l":1.36,"mmol_l":9.25076,"optional":"no"},{"recipe No.":3,"compound":"Vitamin B12","compound_id":18,"amount":0.5,"unit":"mg","g_l":0.0005,"mmol_l":0.000368895,"optional":"no"},{"recipe No.":4,"compound":"Agar","compound_id":3,"attribute":"Difco","amount":8,"unit":"g","g_l":8,"mmol_l":23.7862,"optional":"no"},{"recipe No.":5,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave)."},{"step":"Adjust pH of medium to 7.2 with KOH before as well as after autoclaving and cooling to 50\u00b0C (use pH-indicator paper)."},{"step":"For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton, 3.0 g; CaCl2 x 2 H2O,  0.7 g; MgSO4 x 7 H2O, 2.0 g) to the vial with freeze dried material."}],"equipment":{"946":"Filter","2968":"Autoclave"},"main solution":"yes"}]}