{"medium":{"id":"J1064","name":"GEOSPOROBACTER MEDIUM","complex_medium":"yes","min_pH":7.8,"max_pH":7.8,"source":"JCM","link":"https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1064"},"solutions":[{"id":5129,"name":"Main sol. 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Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0."}],"equipment":{"1891":"Autoclave","3395":"Anaerobic workbench","3719":"Filter"},"main solution":"yes"},{"id":3861,"name":"Trace vitamins","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.00818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.00453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.0486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":5,"unit":"mg","g_l":0.005,"mmol_l":0.0148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":5,"unit":"mg","g_l":0.005,"mmol_l":0.0132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":5,"unit":"mg","g_l":0.005,"mmol_l":0.0406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium pantothenate","compound_id":518,"amount":5,"unit":"mg","g_l":0.005,"mmol_l":0.0104925,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":0.1,"unit":"mg","g_l":0.0001,"mmol_l":7.3779e-5,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":5,"unit":"mg","g_l":0.005,"mmol_l":0.0364591,"optional":"no"},{"recipe No.":10,"compound":"Lipoic acid","compound_id":368,"amount":5,"unit":"mg","g_l":0.005,"mmol_l":0.024233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":3909,"name":"Trace element solution","recipe":[{"recipe No.":1,"compound":"Na2-EDTA","compound_id":34,"amount":0.64,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":6.2,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"MnSO4 x n H2O","compound_id":1000,"amount":0.55,"unit":"g","optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.1,"unit":"g","optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":0.17,"unit":"g","optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.13,"unit":"g","optional":"no"},{"recipe No.":8,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.18,"unit":"g","optional":"no"},{"recipe No.":9,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":0.05,"unit":"g","optional":"no"},{"recipe No.":10,"compound":"AlK(SO4)2 x 12 H2O","compound_id":908,"amount":0.018,"unit":"g","optional":"no"},{"recipe No.":11,"compound":"H3BO3","compound_id":37,"amount":0.01,"unit":"g","optional":"no"},{"recipe No.":12,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.011,"unit":"g","optional":"no"},{"recipe No.":13,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.025,"unit":"g","optional":"no"}],"steps":[{"step":"Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml."}],"main solution":"no"}]}