{"medium":{"id":"J763","name":"ALKALINE TRYPTONE SOYA BROTH MEDIUM","complex_medium":"yes","min_pH":8.8,"max_pH":8.8,"source":"JCM","link":"https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=763"},"solutions":[{"id":4703,"name":"Main sol. J763","volume":1000,"recipe":[{"recipe No.":1,"compound":"Trypticase soy broth","compound_id":99,"attribute":"Oxoid","amount":30,"unit":"g","g_l":30,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Mix components thoroughly and autoclave. After cooling, adjust pH to 8.5 - 9.0 with sterile 20% sodium carbonate solution (autoclaved)."},{"step":"For preparation of solid medium, add 20.0 g/L agar."}],"equipment":{"3727":"Autoclave"},"main solution":"yes"}]}