Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """Baker's yeast""",2.5,g,2.5, """CaCl2 x 2 H2O""",1.36,g,1.36,9.25076 """Vitamin B12""",0.5,mg,0.0005,0.000368895 """Agar""",8,g,8,23.7862 """Distilled water""",1000,ml,, """1. Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave).""" """2. Adjust pH of medium to 7.2 with KOH before as well as after autoclaving and cooling to 50°C (use pH-indicator paper).""" """3. For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton, 3.0 g; CaCl2 x 2 H2O, 0.7 g; MgSO4 x 7 H2O, 2.0 g) to the vial with freeze dried material."""