Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """Solution A""",950,ml,, """Solution B""",30,ml,, """Solution C""",10,ml,, """Solution D""",1,ml,, """Solution E""",10,ml,, """1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.0 - 7.2, if necessary."""