Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """air-dried garden soil""",400,g,400, """Tap water""",1000,ml,, """Agar""",15,g,15,44.5991 """1. Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize."""