{
    "id": 19,
    "name": "Main sol. 14",
    "volume": 1011,
    "recipe": [
        {
            "recipe No.": 1,
            "compound": "Yeast extract",
            "compound_id": 16,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 5,
            "unit": "g",
            "g_l": 4.9456,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 2,
            "compound": "K2HPO4",
            "compound_id": 10,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 10,
            "unit": "g",
            "g_l": 9.8912,
            "mmol_l": 56.7885,
            "optional": "no"
        },
        {
            "recipe No.": 3,
            "compound": null,
            "compound_id": null,
            "solution": "Trace element solution SL-4",
            "solution_id": 20,
            "condition": null,
            "attribute": null,
            "amount": 10,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 4,
            "compound": "Pyridoxine hydrochloride",
            "compound_id": 519,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "0.1% w/v",
            "amount": 1,
            "unit": "ml",
            "g_l": 0.00098912,
            "mmol_l": 0.00480996,
            "optional": "no"
        },
        {
            "recipe No.": 5,
            "compound": "Na-thioglycolate",
            "compound_id": 30,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.75,
            "unit": "g",
            "g_l": 0.74184,
            "mmol_l": 6.50172,
            "optional": "no"
        },
        {
            "recipe No.": 6,
            "compound": "Sodium resazurin",
            "compound_id": 1852,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "0.1% w/v",
            "amount": 0.5,
            "unit": "ml",
            "g_l": 0.00049456,
            "mmol_l": 0.00196902,
            "optional": "no"
        },
        {
            "recipe No.": 7,
            "compound": "Na2CO3",
            "compound_id": 32,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "g",
            "g_l": 0.98912,
            "mmol_l": 9.33237,
            "optional": "no"
        },
        {
            "recipe No.": 8,
            "compound": "D-Fructose",
            "compound_id": 33,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 5,
            "unit": "g",
            "g_l": 4.9456,
            "mmol_l": 27.4511,
            "optional": "no"
        },
        {
            "recipe No.": 9,
            "compound": "Agar",
            "compound_id": 3,
            "solution": null,
            "solution_id": null,
            "condition": "for solid medium",
            "attribute": "",
            "amount": 15,
            "unit": "g",
            "g_l": 14.8368,
            "mmol_l": null,
            "optional": "yes"
        },
        {
            "recipe No.": 10,
            "compound": "Distilled water",
            "compound_id": 4,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1000,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        }
    ],
    "steps": [
        {
            "recipe No.": null,
            "step": "Dissolve ingredients (except carbonate and fructose) and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 8.0."
        }
    ],
    "equipment": {
        "354": "Autoclave",
        "1184": "Hungate tubes",
        "3943": "Gassing station"
    },
    "Sol. No.": null,
    "main solution": "no"
}