Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """NaNO3""",1,g,1,11.7654 """NaHCO3""",1,g,1,11.9038 """Na2S2O3 x 5 H2O""",1,g,1,4.02924 """Sulfur powder""",3,g,3,11.6959 """Wolin's vitamin solution""",10,ml,, """Modified Wolin's mineral solution II""",10,ml,, """Synthetic seawater""",1000,ml,, """1. Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding the vitamins and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, the NaHCO3 and vitamin solution.""" """2. Increase the 80% H2 + 20% CO2 gas phase pressure to 300 kPa. The final pH is 7.0."""