Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """Solution A""",943,ml,, """Solution B""",13,ml,, """Solution C""",20,ml,, """Solution D""",1,ml,, """Solution E""",10,ml,, """Solution F""",10,ml,, """Solution G""",10,ml,, """1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Solutions B, C, D and G are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions E and F are prepared under 100% N2 gas atmosphere and autoclaved. To complete the medium add appropriate amounts of solutions B to G to the sterile solution A in the sequence indicated and let medium equilibrate overnight. Adjust pH of complete medium to 5.7, if necessary.""" """2. After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.""" """3. Note: The complete medium is not stable and can be stored only for a short time."""