Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """Hutner's salts""",20,ml,, """Tris-HCl buffer""",50,ml,,5 """Gelrite""",8,g,8.33333, """MgCl2""",1,g,1.04167,10.9407 """Sea water""",700,ml,, """Distilled water""",190,ml,, """1. Adjust pH to 7.5, autoclave at 121°C for 20 min."""