Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """Solution A""",952,ml,, """Solution B""",30,ml,, """Solution C""",10,ml,, """Solution D""",1,ml,, """Solution E""",13,ml,, """1. Solution A is sparged with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.""" """2. After inoculation add 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture."""