{
    "id": 6282,
    "name": "NaHCO3 buffer (1M)",
    "volume": 50,
    "recipe": [
        {
            "recipe No.": 2,
            "compound": "NaHCO3",
            "compound_id": 55,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 4.2,
            "unit": "g",
            "g_l": 84,
            "mmol_l": 999.917,
            "optional": "no"
        },
        {
            "recipe No.": 3,
            "compound": "Double distilled water",
            "compound_id": 1021,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 50,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        }
    ],
    "steps": [
        {
            "recipe No.": null,
            "step": "Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 \u00b5m) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 \u00b0C. Avoid high headspace to liquid ratios to ensure stable concentration."
        }
    ],
    "equipment": {
        "5166": "Filter"
    },
    "Sol. No.": null,
    "main solution": "no"
}