Compound,Amount,Unit,"Amount [g/L]","Conc. [mM]" """Columbia agar base""",1000,ml,, """Activated charcoal""",2,g,2, """Horse blood""",4,%,40, """1. Add 2 g/l activated charcoal to the columbia agar base solution before autoclaving. Add 4% horse blood after cooling to 50°C. Swirl agar during suspending. To prevent dry agar surface, spread about 1 ml of sterile medium or water over the agar plates and briefly allow to soak into the agar before inoculation. Incubate Neisseria strains in a humid microaerobic atmosphere. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1."""