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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: (NH4)2S4

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J826 MODIFIED CM3 MEDIUM

Compound Conc. [g/L] Conc. [mM]
K2HPO4 1.996 11.46
Yeast extract 1.996 -
KH2PO4 1.497 11
(NH4)2S4 1.297 -
Resazurin 0.998 4.354
MgCl2 x 6 H2O 0.499 2.454
L-Cysteine HCl x H2O 0.499 -
CaCl2 x 2 H2O 0.075 0.509
FeCl2 x 4 H2O 0.001 0.008
FeSO4 x 7 H2O 0.001 0.004
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 -
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Distilled water - -
HCl - -
Glucose - -
Solutions:
  • 1 ml FeCl2 solution
  • 1 ml Trace element solution
Gas phase:
  • 2 % CO2