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Current criteria: 2-(N-Morpholino)ethanesulfonic acid

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1094 METHANOSPHAERULA (PEAT) MEDIUM

Compound Conc. [g/L] Conc. [mM]
2-(N-Morpholino)ethanesulfonic acid 1.936 9.918
Na-acetate 0.407 4.963
NaOH 0.199 4.966
TiCl3 0.082 0.531
2-Mercaptoethanesulfonic acid 0.079 0.559
Na2-EDTA 0.037 0.11
NH4Cl 0.027 0.498
KH2PO4 0.014 0.099
Na2S x 9 H2O 0.01 0.04
AlK(SO4)2 x 12 H2O 0.003 0.007
CaCl2 x 2 H2O 0.002 0.016
MgSO4 x 7 H2O 0.002 0.006
KCl 0.001 0.02
FeCl2 x 4 H2O 0.001 0.007
Pyridoxine hydrochloride 9.9e-5 4.8e-4
ZnCl2 7.4e-5 5.5e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
MnSO4 x 4 H2O 2.6e-5 1.2e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
CoCl2 x 6 H2O 2.4e-5 1.8e-4
Na2Mo4 x 2 H2O 2.4e-5 -
Biotin 2.0e-5 8.1e-5
Folic acid 2.0e-5 4.5e-5
H3BO3 1.9e-5 3.1e-4
CuSO4 x 5 H2O 8.9e-6 3.6e-5
Vitamin B12 9.9e-7 7.3e-7
Tris-HCl buffer - 0.093
Na3-NTA x H2O - 0.031
Distilled water - -
Solutions:
  • 943 ml Solution A
  • 13 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
  • 10 ml Solution G
  • 1 ml Trace element solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 40 % H2
  • 40 % N2
  • 20 % CO2