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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: AlK(SO4)2

Your search received 395 results.

135 ACETOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 9.794 116.589
D-Fructose 9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135a ACETOBACTERIUM MEDIUM (MARINE)

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.897 58.295
Yeast extract 1.959 -
Ethylene glycol 1.224 19.725
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135b ACETOBACTERIUM DEHALOGENANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.988 -
Na2CO3 1.491 14.068
NH4Cl 0.994 18.584
Syringic acid 0.895 -
L-Cysteine HCl x H2O 0.497 2.83
K2HPO4 0.447 2.568
KH2PO4 0.328 2.41
MgSO4 x 7 H2O 0.06 0.242
Nitrilotriacetic acid 0.03 0.156
NaCl 0.02 0.34
MnSO4 x H2O 0.01 0.059
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.014
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 6.0e-4 0.005
Sodium resazurin 5.0e-4 0.002
AlK(SO4)2 x 12 H2O 4.0e-4 8.4e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
  • 15 ml Na-syringate solution (0.3 M)
Gas phase:
  • 80 % N2
  • 20 % CO2

135c MOORELLA THERMOACETICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Fructose
optional 		9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
NaHCO3 0.979 11.659
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

141 METHANOGENIUM MEDIUM (H2/CO2)

Compound Conc. [g/L] Conc. [mM]
NaCl 17.769 304.055
NaHCO3 4.936 58.755
MgCl2 x 6 H2O 3.949 19.423
MgSO4 x 7 H2O 3.406 13.818
Trypticase peptone 1.974 -
Yeast extract 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
CaCl2 x 2 H2O 0.138 0.94
K2HPO4 0.138 0.793
Nitrilotriacetic acid 0.015 0.077
MnSO4 x H2O 0.005 0.029
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.011
ZnSO4 x 7 H2O 0.002 0.006
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 4.9e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
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Solutions:
  • 10 ml Wolin's vitamin solution
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % H2
  • 20 % CO2

141a METHANOCALDOCOCCUS LAUENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3.949 19.423
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
K2HPO4 0.138 0.793
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
NaCl 0.01 0.169
MnSO4 x H2O 0.005 0.029
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.011
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 4.9e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

141b METHANOCULLEUS SP. MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.936 58.755
MgCl2 x 6 H2O 3.949 19.423
Yeast extract 1.974 -
Trypticase peptone 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
K2HPO4 0.138 0.793
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
NaCl 0.01 0.169
MnSO4 x H2O 0.005 0.029
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.011
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 4.9e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

141c METHANOCOCCOIDES MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.936 58.755
Trimethylammonium chloride 4.936 51.646
MgCl2 x 6 H2O 3.949 19.423
Yeast extract 1.974 -
Trypticase peptone 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
K2HPO4 0.138 0.793
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
NaCl 0.01 0.169
MnSO4 x H2O 0.005 0.029
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.011
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 4.9e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

161 METHANOFOLLIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.998 23.784
Yeast extract 0.999 -
Trypticase 0.999 -
L-Cysteine HCl x H2O 0.5 2.844
Na2S x 9 H2O 0.5 2.08
(NH4)2SO4 0.3 2.268
KH2PO4 0.3 2.202
K2HPO4 0.3 1.721
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
FeSO4 x 7 H2O 0.002 0.007
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
Sodium resazurin 5.0e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
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Solutions:
  • 300 ml Clarified rumen fluid
  • 10 ml Modified Wolin's mineral solution
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

165 ACETIVIBRIO CELLULOLYTICUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 2.997 8.755
Cellulose
optional 		2.997 -
Na2CO3 1.499 14.138
(NH4)2SO4 0.45 3.402
K2HPO4 0.292 1.678
L-Cysteine HCl x H2O 0.25 1.422
Na2S x 9 H2O 0.25 1.04
KH2PO4 0.18 1.321
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
Sodium resazurin 5.0e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
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Solutions:
  • 75 ml Mineral solution 1
  • 75 ml Mineral solution 2
  • 20 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Wolin's vitamin solution (10x)
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % N2
  • 20 % CO2
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