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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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29a MEDIUM FOR CHLOROBIUM FERROOXIDANS
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| FeSO4 | 1.519 | 10 |
| NaHCO3 | 1.5 | 17.856 |
| MgSO4 x 7 H2O | 0.52 | 2.11 |
| Ammonium chloride | 0.364 | 6.805 |
| KCl | 0.364 | 4.883 |
| KH2PO4 | 0.364 | 2.675 |
| Ammonium acetate | 0.26 | 3.373 |
| Pyruvic acid sodium salt | 0.26 | 2.363 |
| Dextrose | 0.26 | 1.312 |
| CaCl2 x 2 H2O | 0.25 | 1.701 |
| Yeast extract | 0.25 | - |
| Acetate | 0.118 | 2 |
| Resazurin | 0.002 | 0.01 |
| HCl | 0.002 | 0.055 |
| FeSO4 x 7 H2O | 0.002 | 0.006 |
| Vitamin B12 | 1.0e-3 | 7.4e-4 |
| H3BO3 | 3.1e-4 | 0.005 |
| CoCl2 x 6 H2O | 2.0e-4 | 0.002 |
| MnCl2 x 4 H2O | 1.0e-4 | 5.3e-4 |
| ZnCl2 | 7.3e-5 | 5.3e-4 |
| Na2MoO4 x 2 H2O | 3.7e-5 | 1.5e-4 |
| NiCl2 x 6 H2O | 2.5e-5 | 1.9e-4 |
| CuCl2 x 2 H2O | 2.1e-6 | 1.2e-5 |
| Bicarbonate | - | 10 |
| Distilled water | - | - |
| H2O | - | - |
Solutions:
- 1 ml Trace element solution SL-10 B
- 460 ml Solution A
- 40 ml Solution B
- 50 ml Solution C
- 450 ml Solution D
- 26 ml Solution E
- 10 ml Solution F