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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Biotin

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11a MODIFIED MEDIUM 11

Compound Conc. [g/L] Conc. [mM]
Glucose 19.8 109.902
Meat extract 9.9 -
Casein peptone 9.9 -
Na-acetate 4.95 60.341
Yeast extract 4.95 -
K2HPO4 1.98 11.368
(NH4)3 citrate 1.98 8.141
Tween 80 0.99 0.756
MgSO4 x 7 H2O 0.198 0.803
MnSO4 x H2O 0.05 0.293
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Nicotinic acid 5.0e-5 4.1e-4
Thiamine-HCl x 2 H2O 5.0e-5 1.3e-4
Riboflavin 5.0e-5 1.3e-4
Calcium pantothenate 5.0e-5 1.0e-4
Biotin 2.0e-5 8.2e-5
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Solutions:
  • 10 ml Vitamin solution

25 RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Mannitol 1.5 8.234
KH2PO4 0.5 3.674
Na-gluconate 0.5 2.292
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
Yeast extract 0.25 -
Sodium thiosulfate pentahydrate 0.2 0.806
CaCl2 x 2 H2O 0.05 0.34
Fe(III) citrate 0.005 0.02
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
p-Aminobenzoic acid 1.0e-4 7.3e-4
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
MnCl2 x 4 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
Biotin 2.0e-5 8.2e-5
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Solutions:
  • 1 ml Trace element solution SL-6

34 CARYOPHANON LATUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Soy peptone 2 -
Yeast extract 2 -
Trypticase 2 -
Na-acetate 1 12.19
K2HPO4 1 5.741
MgSO4 x 7 H2O 0.27 1.095
Na glutamate 0.1 0.591
Thiamine-HCl x 2 H2O 2.0e-4 -
Biotin 5.0e-5 2.0e-4
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52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
KH2PO4 0.23 1.69
MgSO4 x 7 H2O 0.2 0.811
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 7.4e-5
p-Aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
D-(+)-biotin 2.0e-5 8.2e-5
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
Vitamin K3 0.047 -
Hemin 0.005 0.007
Resazurin 9.5e-4 0.004
p-Aminobenzoic acid 2.4e-4 0.002
alpha-lipoic acid 2.4e-4 0.001
Pantothenic acid 2.4e-4 0.001
Thiamine-HCl x 2 H2O 2.4e-4 6.3e-4
Riboflavin 2.4e-4 6.3e-4
Vitamin B12 2.4e-4 1.7e-4
Nicotine amide 1.2e-4 9.7e-4
Nicotinic acid 1.2e-4 9.6e-4
Biotin 9.5e-5 3.9e-4
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
Haemin 0.003 0.004
Sodium resazurin 5.9e-4 0.002
Pyridoxine hydrochloride 1.2e-4 5.7e-4
Nicotinic acid 5.9e-5 4.8e-4
p-Aminobenzoic acid 5.9e-5 4.3e-4
(DL)-alpha-Lipoic acid 5.9e-5 2.8e-4
Thiamine HCl 5.9e-5 1.7e-4
Riboflavin 5.9e-5 1.6e-4
Calcium D-(+)-pantothenate 5.9e-5 -
Biotin 2.3e-5 9.6e-5
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
Casamino acids 0.16 -
Yeast extract 0.16 -
Tryptone peptone 0.16 -
Ascorbic acid 0.1 0.568
L-Glutamine 0.07 0.479
L-Histidin 0.06 0.387
L-Serin 0.05 0.476
Vitamin K3 0.05 -
Resazurin 1.0e-3 0.004
Hemin 5.0e-4 7.7e-4
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
Thiamine-HCl x 2 H2O 2.5e-4 6.7e-4
Riboflavin 2.5e-4 6.6e-4
Vitamin B12 2.5e-4 1.8e-4
Nicotine amide 1.3e-4 0.001
Nicotinic acid 1.3e-4 0.001
Biotin 1.0e-4 4.1e-4
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

81 MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Compound Conc. [g/L] Conc. [mM]
Agar 20.101 59.764
Na2HPO4 x 2 H2O 2.915 20.531
KH2PO4 2.312 16.986
NH4Cl 1.005 18.789
MgSO4 x 7 H2O 0.503 2.039
Ferric ammonium citrate 0.05 0.192
CaCl2 x 2 H2O 0.01 0.068
NaVO3 x H2O 0.005 0.036
Nicotinic acid 0.003 0.02
Pyridoxine hydrochloride 0.003 0.012
Calcium pantothenate 0.003 0.005
Thiamine-HCl x 2 H2O 0.003 -
H3BO3 0.002 0.024
CoCl2 x 6 H2O 0.001 0.008
ZnSO4 x 7 H2O 5.0e-4 0.002
Riboflavin 5.0e-4 0.001
Na2MoO4 x 2 H2O 1.5e-4 6.2e-4
MnCl2 x 4 H2O 1.5e-4 -
NiCl2 x 6 H2O 1.0e-4 7.8e-4
CuCl2 x 2 H2O 5.0e-5 2.9e-4
Vitamin B12 5.0e-5 3.7e-5
Folic acid 1.0e-5 2.3e-5
Biotin 5.0e-6 2.1e-5
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Solutions:
  • 5 ml Standard vitamin solution
  • 50 ml Solution A
  • 920 ml Solution B
  • 20 ml Solution C
  • 5 ml Trace element solution SL-6

81a THIOMONAS ISLANDICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 2.868 20.206
K2HPO4 2.275 13.061
NH4Cl 0.989 18.492
NaHCO3 0.495 5.887
MgSO4 x 7 H2O 0.495 2.007
Ferric ammonium citrate 0.049 -
CaCl2 x 2 H2O 0.01 0.067
NaVO3 x H2O 0.005 0.035
H3BO3 0.001 0.024
CoCl2 x 6 H2O 9.9e-4 0.008
ZnSO4 x 7 H2O 4.9e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
MnCl2 x 4 H2O 1.5e-4 7.5e-4
Na2MoO4 x 2 H2O 1.5e-4 6.1e-4
NiCl2 x 6 H2O 9.9e-5 7.6e-4
Calcium pantothenate 9.9e-5 2.1e-4
Vitamin B12 9.9e-5 7.3e-5
p-Aminobenzoic acid 7.9e-5 5.8e-4
CuCl2 x 2 H2O 4.9e-5 2.9e-4
D-(+)-biotin 2.0e-5 8.1e-5
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Solutions:
  • 5 ml Trace element solution SL-6
  • 1 ml Seven vitamins solution
Gas phase:
  • 53 % H2
  • 27 % N2
  • 20 % CO2

87a CHLOROFLEXUS AGGREGANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glycyl-glycine 0.952 -
Yeast extract 0.952 -
NaNO3 0.476 5.603
NaCl 0.095 1.63
KNO3 0.095 0.942
Na2HPO4 x 2 H2O 0.095 0.671
MgSO4 x 7 H2O 0.095 0.386
CaCl2 x 2 H2O 0.048 0.324
Na3-EDTA 0.002 -
Nicotinic acid 9.5e-4 0.008
Thiamine-HCl x 2 H2O 9.5e-4 -
p-Aminobenzoic acid 4.8e-4 0.003
Pyridoxine hydrochloride 4.8e-4 0.002
Folic acid 4.8e-4 0.001
Calcium pantothenate 4.8e-4 1.0e-3
H3BO3 2.9e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
ZnSO4 x 7 H2O 9.5e-5 3.3e-4
Biotin 4.8e-5 1.9e-4
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Solutions:
  • 1 ml Trace element solution SL-6
  • 1 ml Vitamin solution CA
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