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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Calcium pantothenate

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11a MODIFIED MEDIUM 11

Compound Conc. [g/L] Conc. [mM]
Glucose 19.8 109.902
Meat extract 9.9 -
Casein peptone 9.9 -
Na-acetate 4.95 60.341
Yeast extract 4.95 -
K2HPO4 1.98 11.368
(NH4)3 citrate 1.98 8.141
Tween 80 0.99 0.756
MgSO4 x 7 H2O 0.198 0.803
MnSO4 x H2O 0.05 0.293
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Nicotinic acid 5.0e-5 4.1e-4
Thiamine-HCl x 2 H2O 5.0e-5 1.3e-4
Riboflavin 5.0e-5 1.3e-4
Calcium pantothenate 5.0e-5 1.0e-4
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Solutions:
  • 10 ml Vitamin solution

52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
KH2PO4 0.23 1.69
MgSO4 x 7 H2O 0.2 0.811
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

81 MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Compound Conc. [g/L] Conc. [mM]
Agar 20.101 59.764
Na2HPO4 x 2 H2O 2.915 20.531
KH2PO4 2.312 16.986
NH4Cl 1.005 18.789
MgSO4 x 7 H2O 0.503 2.039
Ferric ammonium citrate 0.05 0.192
CaCl2 x 2 H2O 0.01 0.068
NaVO3 x H2O 0.005 0.036
Nicotinic acid 0.003 0.02
Pyridoxine hydrochloride 0.003 0.012
Calcium pantothenate 0.003 0.005
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Solutions:
  • 5 ml Standard vitamin solution
  • 50 ml Solution A
  • 920 ml Solution B
  • 20 ml Solution C
  • 5 ml Trace element solution SL-6

81a THIOMONAS ISLANDICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 2.868 20.206
K2HPO4 2.275 13.061
NH4Cl 0.989 18.492
NaHCO3 0.495 5.887
MgSO4 x 7 H2O 0.495 2.007
Ferric ammonium citrate 0.049 -
CaCl2 x 2 H2O 0.01 0.067
NaVO3 x H2O 0.005 0.035
H3BO3 0.001 0.024
CoCl2 x 6 H2O 9.9e-4 0.008
ZnSO4 x 7 H2O 4.9e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
MnCl2 x 4 H2O 1.5e-4 7.5e-4
Na2MoO4 x 2 H2O 1.5e-4 6.1e-4
NiCl2 x 6 H2O 9.9e-5 7.6e-4
Calcium pantothenate 9.9e-5 2.1e-4
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Solutions:
  • 5 ml Trace element solution SL-6
  • 1 ml Seven vitamins solution
Gas phase:
  • 53 % H2
  • 27 % N2
  • 20 % CO2

87a CHLOROFLEXUS AGGREGANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glycyl-glycine 0.952 -
Yeast extract 0.952 -
NaNO3 0.476 5.603
NaCl 0.095 1.63
KNO3 0.095 0.942
Na2HPO4 x 2 H2O 0.095 0.671
MgSO4 x 7 H2O 0.095 0.386
CaCl2 x 2 H2O 0.048 0.324
Na3-EDTA 0.002 -
Nicotinic acid 9.5e-4 0.008
Thiamine-HCl x 2 H2O 9.5e-4 -
p-Aminobenzoic acid 4.8e-4 0.003
Pyridoxine hydrochloride 4.8e-4 0.002
Folic acid 4.8e-4 0.001
Calcium pantothenate 4.8e-4 1.0e-3
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Solutions:
  • 1 ml Trace element solution SL-6
  • 1 ml Vitamin solution CA

92a MODIFIED MEDIUM 92

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 29.7 -
Agar 14.85 44.153
Yeast extract 2.97 -
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Nicotinic acid 5.0e-5 4.1e-4
Thiamine-HCl x 2 H2O 5.0e-5 1.3e-4
Riboflavin 5.0e-5 1.3e-4
Calcium pantothenate 5.0e-5 1.0e-4
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Solutions:
  • 10 ml Vitamin solution

142 THIOMICROSPIRA PELOPHILA MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 24.876 425.661
Na2S2O3 x 5 H2O 4.975 20.046
MgSO4 x 7 H2O 1.493 6.056
(NH4)2SO4 0.995 7.53
K2HPO4 0.498 2.856
Na2-EDTA 0.01 0.03
ZnSO4 x 7 H2O 0.004 0.015
Bromothymol blue 0.004 0.006
CaCl2 x 2 H2O 0.001 0.007
MnCl2 x 4 H2O 0.001 0.005
FeSO4 x 7 H2O 1.0e-3 0.004
CoCl2 x 6 H2O 3.2e-4 0.002
CuSO4 x 5 H2O 3.1e-4 0.001
Pyridoxine hydrochloride 3.0e-4 0.001
(NH4)6Mo7O24 x 4 H2O 2.2e-4 1.8e-4
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
Calcium pantothenate 1.0e-4 2.1e-4
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Solutions:
  • 0.2 ml Trace element solution (Vishniac & Santer, 1957)
  • 1 ml Seven vitamins solution
Gas phase:
  • 75 % N2
  • 15 % CO2
  • 10 % O2

142a THIOMICRORHABDUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
Na2S2O3 x 5 H2O 5 20.146
MgSO4 x 7 H2O 1.5 6.086
(NH4)2SO4 1 7.568
K2HPO4 0.5 2.871
CaCl2 x 2 H2O 0.42 2.857
Bromothymol blue 0.004 0.006
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Seven vitamins solution

148 DESULFUROMONAS SP. MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 1.5 14.153
Fumaric acid 1.5 12.923
KH2PO4 1 7.348
NH4Cl 0.5 9.348
Na-acetate 0.5 6.095
Na2S x 9 H2O 0.5 2.082
MgSO4 x 7 H2O 0.4 1.623
CaCl2 x 2 H2O 0.1 0.68
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 100 % N2

149 MEGADESULFOVIBRIO MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2 14.081
Na-L-lactate 2 -
Na2CO3 1 9.435
KH2PO4 1 7.348
NH4Cl 0.5 9.348
MgSO4 x 7 H2O 0.4 1.623
Na2S x 9 H2O 0.3 1.249
CaCl2 x 2 H2O 0.1 0.68
Sodium resazurin 5.0e-4 0.002
H3BO3 3.0e-4 0.005
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
CoCl2 x 6 H2O 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
Calcium pantothenate 1.0e-4 2.1e-4
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Solutions:
  • 970 ml Solution A
  • 20 ml Solution B
  • 1 ml Solution C
  • 10 ml Solution D
  • 1 ml Trace element solution SL-6
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2
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