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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: Cellobiose

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110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
Gas phase:
  • 100 % N2

110a CMC MEDIUM (N2/CO2)

Compound Conc. [g/L] Conc. [mM]
Ground beef 495.049 -
Casitone 29.703 -
K2HPO4 4.951 28.422
Yeast extract 4.951 -
D-Glucose 3.96 21.983
Na2CO3 1.485 14.012
D-Maltose 0.99 2.893
Cellobiose 0.99 2.892
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Solutions:
  • 1000 ml Meat filtrate
  • 10 ml Sugar mix
Gas phase:
  • 80 % N2
  • 20 % CO2

122 ACETIVIBRIO MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellulose
optional
10 -
Na2-ß-glycerophosphate x 5 H2O 6 19.601
K2HPO4 5.5 31.577
Cellobiose 5 14.607
Yeast extract 4.5 -
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Gas phase:
  • 80 % N2
  • 20 % CO2

140 RUMINICLOSTRIDIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Na2CO3 2.5 23.588
Yeast extract 1.2 -
NaCl 1 17.112
(NH4)2SO4 0.5 3.784
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Solutions:
  • 300 ml Sludge fluid
  • 300 ml Clarified rumen fluid
Gas phase:
  • 100 % CO2

160 CYTOPHAGA HUTCHINSONII MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Cellobiose 5 14.607
Casitone 3 -
CaCl2 x 2 H2O 1.36 9.251
Yeast extract 1 -
Distilled water - -

165 ACETIVIBRIO CELLULOLYTICUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 2.997 8.755
Cellulose
optional
2.997 -
Na2CO3 1.499 14.138
(NH4)2SO4 0.45 3.402
K2HPO4 0.292 1.678
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Solutions:
  • 75 ml Mineral solution 1
  • 75 ml Mineral solution 2
  • 20 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Wolin's vitamin solution (10x)
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % N2
  • 20 % CO2

212a TREPONEMA RECTALE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.778 19.556
Trehalose 1.884 5.504
Maltose x H2O 1.786 5.217
Acetic acid 1.763 29.355
Cellobiose 1.688 4.93
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Solutions:
  • 917 ml Solution A
  • 30 ml Solution B
  • 4 ml Solution C
  • 4 ml Solution D
  • 30 ml Solution E
  • 1 ml Solution F
  • 2 ml Solution G
  • 10 ml Solution H
  • 10 ml Solution I
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Clarified rumen fluid
  • 1 ml Vitamin K1 solution
  • 3.1 ml Volatile fatty acid mixture
  • 30 ml Sugar mix
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

255 CLOSTRIDIUM (GS) MEDIUM

Compound Conc. [g/L] Conc. [mM]
Morpholinopropane sulfonic acid 9.98 47.692
Yeast extract 5.988 -
Cellobiose 4.99 14.578
Urea 1.996 33.236
K2HPO4 x 3 H2O 0.998 5.73
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Gas phase:
  • 100 % N2

289 RUMINICLOSTRIDIUM PAPYROSOLVENS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Cellulose
optional
5 -
K2HPO4 1.65 9.473
NH4Cl 1 18.695
NaCl 0.9 15.4
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Solutions:
  • 150 ml Mineral salt solution
Gas phase:
  • 100 % N2

315 BACTEROIDES CELLULOSOLVENS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Cellulose 5 -
NaHCO3 2 23.808
NH4Cl 0.68 12.713
K2HPO4 0.3 1.722
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Solutions:
  • 10 ml Trace element solution
  • 10 ml Vitamin solution
Gas phase:
  • 80 % N2
  • 20 % CO2
Show 1 to 10 (58 total)