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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: CoCl2 x 6 H2O

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14 CLOSTRIDIUM FORMICACETICUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.837 -
K2HPO4 9.891 56.789
D-Fructose 4.946 27.451
Yeast extract 4.946 -
Na2CO3 0.989 9.332
Na-thioglycolate 0.742 6.502
Na2-EDTA 0.005 0.015
H3BO3 0.003 0.048
CoCl2 x 6 H2O 0.002 0.015
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Solutions:
  • 10 ml Trace element solution SL-4
Gas phase:
  • 100 % N2

25 RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Mannitol 1.5 8.234
KH2PO4 0.5 3.674
Na-gluconate 0.5 2.292
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
Yeast extract 0.25 -
Sodium thiosulfate pentahydrate 0.2 0.806
CaCl2 x 2 H2O 0.05 0.34
Fe(III) citrate 0.005 0.02
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
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Solutions:
  • 1 ml Trace element solution SL-6

26 ACID RHODOSPIRILLACEAE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
L-Cysteine HCl 0.3 1.903
Yeast extract 0.2 -
CaCl2 x 2 H2O 0.05 0.34
Resazurin 0.005 0.022
Fe(III) citrate 0.005 0.02
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
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Solutions:
  • 1 ml Trace element solution SL-6
Gas phase:
  • 100 % N2

27 RHODOSPIRILLACEAE MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
L-Cysteine HCl 0.3 1.903
Yeast extract 0.3 -
CaCl2 x 2 H2O 0.05 0.34
Resazurin 0.005 0.022
Fe(III) citrate 0.005 -
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
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Solutions:
  • 1 ml Trace element solution SL-6

28 PFENNIG'S MEDIUM I

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

28a THIORHODOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
NaHCO3 1.5 17.856
MgCl2 x 6 H2O 1 4.919
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Sodium Thiosulfate 0.5 3.162
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

28b THIORHODOCOCCUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
MgCl2 x 6 H2O 2.5 12.297
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Na2S2O3 x 5 H2O 0.5 2.015
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

29 PFENNIG'S MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
KH2PO4 0.364 2.675
Ammonium acetate 0.26 3.373
Pyruvic acid sodium salt 0.26 2.363
Dextrose 0.26 1.312
CaCl2 x 2 H2O 0.25 1.701
Yeast extract 0.25 -
Resazurin 0.002 0.01
HCl 0.002 0.055
FeSO4 x 7 H2O 0.002 0.006
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.1e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
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Solutions:
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F
  • 1 ml Trace element solution SL-10 B

29a MEDIUM FOR CHLOROBIUM FERROOXIDANS

Compound Conc. [g/L] Conc. [mM]
FeSO4 1.519 10
NaHCO3 1.5 17.856
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
KH2PO4 0.364 2.675
Ammonium acetate 0.26 3.373
Pyruvic acid sodium salt 0.26 2.363
Dextrose 0.26 1.312
CaCl2 x 2 H2O 0.25 1.701
Yeast extract 0.25 -
Acetate 0.118 2
Resazurin 0.002 0.01
HCl 0.002 0.055
FeSO4 x 7 H2O 0.002 0.006
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.1e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
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Solutions:
  • 1 ml Trace element solution SL-10 B
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F

39 PFENNIG'S MEDIUM I WITH YEAST EXTRACT

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Yeast extract 0.5 -
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B
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