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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: CoSO4 x 7 H2O

Your search received 384 results.

88 SULFOLOBUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.287 9.741
Yeast extract 0.99 -
KH2PO4 0.277 2.037
MgSO4 x 7 H2O 0.248 1.004
CaCl2 x 2 H2O 0.069 0.471
FeCl3 x 6 H2O 0.02 0.073
Na2B4O7 x 10 H2O 0.004 -
MnCl2 x 4 H2O 0.002 0.009
ZnSO4 x 7 H2O 2.2e-4 7.6e-4
CuCl2 x 2 H2O 5.0e-5 2.9e-4
VOSO4 x 2 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
CoSO4 x 7 H2O 9.9e-6 6.4e-5
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Solutions:
  • 10 ml Allen's trace element solution

88a SULFOLOBUS MEDIUM (ANAEROBIC)

Compound Conc. [g/L] Conc. [mM]
Sulfur 10 311.818
(NH4)2SO4 1.3 9.838
Na2S x 9 H2O 0.5 2.082
Yeast extract 0.5 -
KH2PO4 0.28 2.058
MgSO4 x 7 H2O 0.25 1.014
CaCl2 x 2 H2O 0.07 0.476
FeCl3 x 6 H2O 0.02 0.074
Na2B4O7 x 10 H2O 0.005 -
MnCl2 x 4 H2O 0.002 0.009
ZnSO4 x 7 H2O 2.2e-4 7.7e-4
CuCl2 x 2 H2O 5.0e-5 2.9e-4
VOSO4 x 2 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
CoSO4 x 7 H2O 1.0e-5 6.5e-5
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Solutions:
  • 10 ml Allen's trace element solution
Gas phase:
  • 100 % N2

88b V24N MEDIUM

Compound Conc. [g/L] Conc. [mM]
Starch 2 -
(NH4)2SO4 1.3 9.838
Sulfur 1 31.182
Na2S x 9 H2O 0.5 2.082
KH2PO4 0.28 2.058
MgSO4 x 7 H2O 0.25 1.014
Yeast extract 0.2 -
CaCl2 x 2 H2O 0.07 0.476
FeCl3 x 6 H2O 0.02 0.074
Na2B4O7 x 10 H2O 0.005 -
MnCl2 x 4 H2O 0.002 -
Resazurin 4.0e-4 0.002
ZnSO4 x 7 H2O 2.2e-4 7.7e-4
CuCl2 x 2 H2O 5.0e-5 2.9e-4
VOSO4 x 2 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
CoSO4 x 7 H2O 1.0e-5 6.5e-5
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Solutions:
  • 10 ml Allen's trace element solution
Gas phase:
  • 100 % N2

88c ZESTOSPHAERA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2 8.058
(NH4)2SO4 1.3 9.838
Tryptone peptone 1 -
Yeast extract 1 -
Na2S x 9 H2O 0.5 2.082
Casamino acids 0.5 -
KH2PO4 0.28 2.058
MgSO4 x 7 H2O 0.25 1.014
CaCl2 x 2 H2O 0.07 0.476
FeCl3 x 6 H2O 0.02 0.074
Na2B4O7 x 10 H2O 0.005 -
MnCl2 x 4 H2O 0.002 -
ZnSO4 x 7 H2O 2.2e-4 7.7e-4
CuCl2 x 2 H2O 5.0e-5 2.9e-4
VOSO4 x 2 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
CoSO4 x 7 H2O 1.0e-5 6.5e-5
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Solutions:
  • 10 ml Allen's trace element solution
Gas phase:
  • 80 % N2
  • 20 % CO2

135 ACETOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 9.794 116.589
D-Fructose 9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135a ACETOBACTERIUM MEDIUM (MARINE)

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.897 58.295
Yeast extract 1.959 -
Ethylene glycol 1.224 19.725
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135b ACETOBACTERIUM DEHALOGENANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.988 -
Na2CO3 1.491 14.068
NH4Cl 0.994 18.584
Syringic acid 0.895 -
L-Cysteine HCl x H2O 0.497 2.83
K2HPO4 0.447 2.568
KH2PO4 0.328 2.41
MgSO4 x 7 H2O 0.06 0.242
Nitrilotriacetic acid 0.03 0.156
NaCl 0.02 0.34
MnSO4 x H2O 0.01 0.059
CoSO4 x 7 H2O 0.004 0.023
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
  • 15 ml Na-syringate solution (0.3 M)
Gas phase:
  • 80 % N2
  • 20 % CO2

135c MOORELLA THERMOACETICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Fructose
optional 		9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
NaHCO3 0.979 11.659
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

141 METHANOGENIUM MEDIUM (H2/CO2)

Compound Conc. [g/L] Conc. [mM]
NaCl 17.769 304.055
NaHCO3 4.936 58.755
MgCl2 x 6 H2O 3.949 19.423
MgSO4 x 7 H2O 3.406 13.818
Trypticase peptone 1.974 -
Yeast extract 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
CaCl2 x 2 H2O 0.138 0.94
K2HPO4 0.138 0.793
Nitrilotriacetic acid 0.015 0.077
MnSO4 x H2O 0.005 0.029
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.011
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Solutions:
  • 10 ml Wolin's vitamin solution
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % H2
  • 20 % CO2

141a METHANOCALDOCOCCUS LAUENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3.949 19.423
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
K2HPO4 0.138 0.793
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
NaCl 0.01 0.169
MnSO4 x H2O 0.005 0.029
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.011
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2
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