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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: CuSO4 x 5 H2O

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68 HALOTHIOBACILLUS NEAPOLITANUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		15 44.599
Na2S2O3 x 5 H2O 10 40.292
KH2PO4 4 29.393
K2HPO4 4 22.965
MgSO4 x 7 H2O 0.8 3.246
NH4Cl 0.4 7.478
Na2-EDTA 0.25 0.744
ZnSO4 x 7 H2O 0.11 0.383
CaCl2 x 2 H2O 0.028 0.188
MnCl2 x 4 H2O 0.025 0.128
FeSO4 x 7 H2O 0.025 0.09
CoCl2 x 6 H2O 0.008 0.062
CuSO4 x 5 H2O 0.008 0.031
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Solutions:
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

69 STARKEYA NOVELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.892 -
Na2HPO4 x 12 H2O 10.524 74.133
Na2S2O3 x 5 H2O 4.975 20.046
KH2PO4 1.489 10.943
NH4Cl 0.298 5.568
Yeast extract 0.298 -
Na2-EDTA 0.249 0.74
ZnSO4 x 7 H2O 0.109 0.381
MgSO4 x 7 H2O 0.1 0.404
CaCl2 x 2 H2O 0.028 0.187
MnCl2 x 4 H2O 0.025 0.127
FeSO4 x 7 H2O 0.025 0.089
CoCl2 x 6 H2O 0.008 0.062
CuSO4 x 5 H2O 0.008 0.031
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Solutions:
  • 900 ml Solution A
  • 50 ml Solution B
  • 5 ml Solution C
  • 50 ml Solution D
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

86 CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Tryptone 1 14.069
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
KNO3 0.103 1.019
Nitrilotriacetic acid 0.1 0.523
MgSO4 x 7 H2O 0.1 0.406
CaSO4 x 2 H2O 0.06 0.348
NaCl 0.008 0.137
MnSO4 x H2O 0.002 0.013
H3BO3 5.0e-4 0.008
ZnSO4 x 7 H2O 5.0e-4 0.002
FeCl3 x 6 H2O 4.7e-4 0.002
CoCl2 x 6 H2O 4.6e-5 3.5e-4
Na2MoO4 x 2 H2O 2.5e-5 1.0e-4
CuSO4 x 5 H2O 2.5e-5 1.0e-4
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86a MODIFIED CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 25 74.332
Peptone 3 -
Monosodium glutamate 1 5.913
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
KNO3 0.103 1.019
Nitrilotriacetic acid 0.1 0.523
MgSO4 x 7 H2O 0.1 0.406
CaSO4 x 2 H2O 0.06 0.348
NaCl 0.008 0.137
MnSO4 x H2O 0.002 0.013
H3BO3 5.0e-4 0.008
ZnSO4 x 7 H2O 5.0e-4 0.002
FeCl3 x 6 H2O 4.7e-4 0.002
CoCl2 x 6 H2O 4.6e-5 3.5e-4
Na2MoO4 x 2 H2O 2.5e-5 1.0e-4
CuSO4 x 5 H2O 2.5e-5 1.0e-4
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125 METHYLOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
KNO3 1 9.891
Na2HPO4 0.23 1.62
MgSO4 x 7 H2O 0.2 0.811
NaH2PO4 0.07 0.583
CaCl2 x 2 H2O 0.02 0.136
FeSO4 x 7 H2O 1.0e-3 0.004
ZnSO4 x 7 H2O 7.0e-5 2.4e-4
H3BO3 1.0e-5 1.6e-4
MoO3 1.0e-5 6.9e-5
MnSO4 x 5 H2O 1.0e-5 4.1e-5
CuSO4 x 5 H2O 5.0e-6 2.0e-5
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135 ACETOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 9.794 116.589
D-Fructose 9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135a ACETOBACTERIUM MEDIUM (MARINE)

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.897 58.295
Yeast extract 1.959 -
Ethylene glycol 1.224 19.725
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135b ACETOBACTERIUM DEHALOGENANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.988 -
Na2CO3 1.491 14.068
NH4Cl 0.994 18.584
Syringic acid 0.895 -
L-Cysteine HCl x H2O 0.497 2.83
K2HPO4 0.447 2.568
KH2PO4 0.328 2.41
MgSO4 x 7 H2O 0.06 0.242
Nitrilotriacetic acid 0.03 0.156
NaCl 0.02 0.34
MnSO4 x H2O 0.01 0.059
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.014
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 6.0e-4 0.005
Sodium resazurin 5.0e-4 0.002
AlK(SO4)2 x 12 H2O 4.0e-4 8.4e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.2e-4
CuSO4 x 5 H2O 2.0e-4 8.0e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
  • 15 ml Na-syringate solution (0.3 M)
Gas phase:
  • 80 % N2
  • 20 % CO2

135c MOORELLA THERMOACETICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Fructose
optional 		9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
NaHCO3 0.979 11.659
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

137 MINERAL LACTATE MEDIUM (low carbon content)

Compound Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 1.13 6.488
NH4Cl 1 18.695
NaCl 1 17.112
KH2PO4 0.88 6.467
Na-lactate 0.5 4.462
MgSO4 x 7 H2O 0.5 2.029
Na2-EDTA 0.06 0.178
ZnSO4 x 7 H2O 0.026 0.092
CaCl2 x 2 H2O 0.007 0.045
MnCl2 x 4 H2O 0.006 0.031
FeSO4 x 7 H2O 0.006 0.022
CoCl2 x 6 H2O 0.002 0.015
CuSO4 x 5 H2O 0.002 0.008
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Solutions:
  • 1.2 ml Trace element solution (Vishniac & Santer, 1957)
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