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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: CuSO4 x 6 H2O
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111 BEIJERINCKIA MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15.79 | 46.946 |
| Glucose | 10.526 | 58.428 |
| K2HPO4 | 0.842 | 4.835 |
| KH2PO4 | 0.211 | 1.547 |
| MgSO4 x 7 H2O | 0.105 | 0.427 |
| FeSO4 x 7 H2O | 0.021 | 0.076 |
| Na2MoO4 x 2 H2O | 0.005 | 0.022 |
| ZnSO4 x 6 H2O | 0.005 | 0.02 |
| CuSO4 x 6 H2O | 0.004 | 0.016 |
| MnSO4 x 6 H2O | 0.002 | 0.008 |
| Distilled water | - | - |
1498 PE MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| K2HPO4 | 1.141 | 6.552 |
| KH2PO4 | 0.68 | 5 |
| Sodium acetate | 0.5 | 6.095 |
| (NH4)2SO4 | 0.5 | 3.784 |
| Sodium Thiosulfate | 0.5 | 3.162 |
| Sodium succinate | 0.5 | 3.085 |
| Na glutamate | 0.5 | 2.957 |
| Yeast extract | 0.5 | - |
| Casamino acids | 0.5 | - |
| MgSO4 x 7 H2O | 0.123 | 0.5 |
| NaCl | 0.117 | 2.002 |
| CaCl2 x 2 H2O | 0.015 | 0.1 |
| FeSO4 x 7 H2O | 0.006 | 0.02 |
| Na3-EDTA | 0.002 | 0.006 |
| Nicotinic acid | 1.0e-3 | 0.008 |
| Thiamine-HCl x 2 H2O | 1.0e-3 | 0.003 |
| MnSO4 x 4 H2O | 5.6e-4 | 0.003 |
| p-Aminobenzoic acid | 5.0e-4 | 0.004 |
| Pyridoxine hydrochloride | 5.0e-4 | 0.002 |
| Folic acid | 5.0e-4 | 0.001 |
| Calcium pantothenate | 5.0e-4 | 0.001 |
| H3BO3 | 1.5e-4 | 0.003 |
| Co(NO3)2 x 6 H2O | 1.5e-4 | 5.0e-4 |
| ZnSO4 x 7 H2O | 1.4e-4 | 5.0e-4 |
| CuSO4 x 6 H2O | 1.3e-4 | 4.7e-4 |
| Na2MoO4 x 2 H2O | 1.2e-4 | 5.0e-4 |
| Biotin | 5.0e-5 | 2.0e-4 |
| Vitamin B12 | 1.0e-5 | 7.4e-6 |
| Distilled water | - | - |
Solutions:
- 5 ml Basal Salts Solution
- 5 ml Phosphate solution (1M)
- 1 ml Vitamin solution CA