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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: DL-Na-lactate
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1049 SELENATE REDUCER MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 22.867 | 391.296 |
| Agar | 14.914 | 44.342 |
| MgCl2 x 6 H2O | 2.983 | 14.671 |
| NaHCO3 | 2.336 | 27.813 |
| Na2SeO4 | 1.766 | 9.348 |
| KCl | 1.293 | 17.337 |
| DL-Na-lactate | 1.114 | 9.937 |
| NH4Cl | 0.497 | 9.294 |
| KH2PO4 | 0.199 | 1.461 |
| CaCl2 x 2 H2O | 0.099 | 0.676 |
| Na2S x 9 H2O | 0.093 | 0.389 |
| HCl | 0.002 | 0.064 |
| FeCl2 x 4 H2O | 0.001 | 0.007 |
| Resazurin | 5.0e-4 | 0.002 |
| 1,4-Naphthoquinone | 1.9e-4 | 0.001 |
| CoCl2 x 6 H2O | 1.8e-4 | 0.001 |
| MnCl2 x 4 H2O | 9.3e-5 | 4.7e-4 |
| Na2WO4 | 7.5e-5 | 2.5e-4 |
| ZnCl2 | 6.5e-5 | 4.8e-4 |
| Hemin | 4.7e-5 | 7.2e-5 |
| Na2MoO4 x 2 H2O | 3.4e-5 | 1.4e-4 |
| Na2SeO3 | 2.8e-5 | 1.6e-4 |
| NiCl2 x 6 H2O | 2.2e-5 | 1.7e-4 |
| H3BO3 | 5.6e-6 | 9.1e-5 |
| p-Aminobenzoic acid | 2.3e-6 | 1.7e-5 |
| alpha-lipoic acid | 2.3e-6 | 1.1e-5 |
| Pantothenic acid | 2.3e-6 | 1.1e-5 |
| Thiamine-HCl x 2 H2O | 2.3e-6 | 6.3e-6 |
| Riboflavin | 2.3e-6 | 6.2e-6 |
| Vitamin B12 | 2.3e-6 | 1.7e-6 |
| CuCl2 x 2 H2O | 1.9e-6 | 1.1e-5 |
| Nicotine amide | 1.2e-6 | 9.6e-6 |
| Nicotinic acid | 1.2e-6 | 9.5e-6 |
| Biotin | 9.3e-7 | 3.8e-6 |
| Folic acid | 9.3e-7 | 2.1e-6 |
| Pyridoxamine hydrochloride | 4.7e-7 | 2.3e-6 |
| Distilled water | - | - |
Solutions:
- 100 ml Solution A
- 1 ml Solution B
- 2.5 ml Solution C
- 1 ml Solution D
- 0.1 ml Solution E
- 0.5 ml Solution F
- 1 ml Solution G
- 1 ml Solution H
- 1 ml Vitamin solution
- 1 ml Trace element solution SL-10