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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Disodium fumarate
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1200 ANAEROMYXOBACTER-MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 4.545 | 54.108 |
| NaCl | 1.006 | 17.208 |
| MgCl2 x 6 H2O | 0.503 | 2.473 |
| Na-acetate | 0.402 | 4.904 |
| Disodium fumarate | 0.364 | 2.272 |
| Show more | ||
Solutions:
- 10 ml Solution A
- 1 ml Solution B
- 0.2 ml Solution C
- 0.2 ml Solution D ("red mix")
- 0.05 ml Solution E (electron acceptor)
- 0.2 ml Solution F: Vitamin solution according to Wolin and coauthors
- 1 ml Trace element solution SL-10 B
- 2 ml Selenite-tungstate solution
J994 MBM MEDIUM 2
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 78.288 | 1339.63 |
| MgSO4 x 7 H2O | 5.9 | 23.938 |
| Sodium acetate x 3 H2O | 1.566 | - |
| KCl | 1.468 | 19.688 |
| NH4Cl | 0.978 | 18.293 |
| CaCl2 x 2 H2O | 0.392 | 2.669 |
| K2HPO4 | 0.391 | 2.247 |
| Nitrilotriacetic acid | 0.015 | 0.077 |
| MnSO4 x n H2O | 0.005 | - |
| CoSO4 x 7 H2O | 9.8e-4 | 0.006 |
| Resazurin | 9.8e-4 | 0.004 |
| FeSO4 x 7 H2O | 9.8e-4 | 0.004 |
| ZnSO4 x 7 H2O | 9.8e-4 | 0.003 |
| H3BO3 | 9.8e-5 | 0.002 |
| Na2MoO4 x 2 H2O | 9.8e-5 | 4.0e-4 |
| CuSO4 x 5 H2O | 9.8e-5 | 3.9e-4 |
| AlK(SO4)2 | 9.8e-5 | 3.8e-4 |
| Pyridoxine hydrochloride | 9.8e-5 | - |
| Nicotinic acid | 4.9e-5 | 4.0e-4 |
| p-Aminobenzoic acid | 4.9e-5 | 3.6e-4 |
| Lipoic acid | 4.9e-5 | 2.4e-4 |
| Riboflavin | 4.9e-5 | 1.3e-4 |
| Calcium pantothenate | 4.9e-5 | 1.0e-4 |
| Thiamine HCl | 4.9e-5 | - |
| Biotin | 2.0e-5 | 8.0e-5 |
| Folic acid | 2.0e-5 | 4.4e-5 |
| Vitamin B12 | 9.8e-7 | 7.2e-7 |
| NaHCO3 | - | - |
| Disodium fumarate | - | - |
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Solutions:
- 10 ml Trace minerals
- 10 ml Trace vitamins
J1062 DESULFUROMONAS CARBONIS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 8.148 | 139.428 |
| Disodium fumarate | 7.407 | 46.285 |
| MgCl2 x 6 H2O | 4.259 | 20.95 |
| KCl | 0.315 | 4.223 |
| NH4Cl | 0.231 | 4.328 |
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Solutions:
- 10 ml Trace element solution
- 10 ml Trace vitamins
J1235 R2A AGAR WITH FUMARATE
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| R2A agar | 18.2 | - |
| Disodium fumarate | 3.2 | 19.995 |
| Distilled water | - | - |