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Current criteria: Disodium sulfide

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1478 NATRANAEROARCHAEUM MEDIUM AARC1

Compound Conc. [g/L] Conc. [mM]
Na2CO3 142.5 1344.49
NaCl 72 1232.03
Sodium formate 3.4 50
K2HPO4 2.5 14.353
Sulfur powder 2 7.797
MgCl2 x 6 H2O 0.244 1.2
Sodium acetate 0.164 2
NH4Cl 0.16 3
(NH4)2SO4 0.125 0.946
NaHCO3 0.105 1.25
Yeast extract 0.02 -
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 1.0e-4 6.2e-4
MnCl2 3.0e-5 2.4e-4
Pyridoxine 2.5e-5 1.5e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
Na2MoO4 x 2 H2O 2.0e-5 8.3e-5
Nicotinic acid 1.3e-5 1.0e-4
PABA 1.3e-5 9.1e-5
Thiamine 1.3e-5 4.7e-5
Riboflavin 1.3e-5 3.3e-5
Calcium pantothenate 1.3e-5 2.6e-5
CuCl2 x 5 H2O 1.0e-5 4.5e-5
Biotin 5.0e-6 2.0e-5
Folic acid 5.0e-6 1.1e-5
B12 1.3e-6 9.2e-7
Disodium sulfide - 1
Distilled water - -
NaOH - -
Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamine mixture
  • 100 ml Acidic thiamine / Ca pantothenate solution
  • 100 ml Basic vitamin solution
  • 100 ml Neutral folic acid / riboflavin solution
  • 100 ml Neutral B12 solution 0.005 g/L

1479 HALODESULFURIARCHAEUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 240 4106.78
HEPES 10 41.964
Sodium formate 3.4 50
K2HPO4 2.5 14.353
Sulfur powder 2 7.797
MgCl2 x 6 H2O 0.61 3
(NH4)2SO4 0.5 3.784
NaHCO3 0.42 5
Yeast extract 0.2 -
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 1.0e-4 6.2e-4
MnCl2 3.0e-5 2.4e-4
Pyridoxine 2.5e-5 1.5e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
Na2MoO4 x 2 H2O 2.0e-5 8.3e-5
Nicotinic acid 1.3e-5 1.0e-4
PABA 1.3e-5 9.1e-5
Thiamin 1.3e-5 4.7e-5
Riboflavin 1.3e-5 3.3e-5
Calcium pantothenate 1.3e-5 2.6e-5
CuCl2 x 5 H2O 1.0e-5 4.5e-5
Biotin 5.0e-6 2.0e-5
Folic acid 5.0e-6 1.1e-5
B12 1.3e-6 9.2e-7
Disodium sulfide - 1
Distilled water - -
Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamin mix (pfennig & Lippert,1966)