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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Ethanol

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78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

93 LACTOBACILLUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
Na-acetate 20 243.802
Glucose 20 111.012
Agar 15 44.599
Casein peptone 10 -
Yeast extract 5 -
Meat extract 2 -
KH2PO4 0.5 3.674
K2HPO4 0.5 2.871
MgSO4 x 7 H2O 0.2 0.811
DL-mevalonic acid 0.03 0.202
FeSO4 x 7 H2O 0.01 0.036
MnSO4 x H2O 0.008 0.044
Tween 80 - -
Ethanol - -
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95 DESULFUROMONAS ACETOXIDANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 19.92 340.868
Na2-DL-malate 2.649 14.88
MgCl2 x 6 H2O 1.992 9.798
NaHCO3 1.843 21.934
Na2SO4 1.763 12.412
KH2PO4 0.996 7.319
MgSO4 x 7 H2O 0.996 4.041
Yeast extract 0.498 -
Na2S x 9 H2O 0.299 1.244
NH4Cl 0.299 5.586
CaCl2 x 2 H2O 0.1 0.677
Na2-EDTA x 2 H2O 0.005 0.015
FeCl2 x 4 H2O 0.001 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Distilled water - -
Ethanol - -
Solutions:
  • 951 ml Solution A
  • 3 ml Solution B
  • 40 ml Solution C
  • 10 ml Solution D
  • 1 ml Trace element solution SL-11
Gas phase:
  • 100 % N2

104 PYG MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
L-Cysteine HCl x H2O 0.5 -
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
Ethanol 0.004 0.082
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution

110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
D-Glucose 4 22.203
Maltose 1 2.921
Cellobiose 1 2.921
Starch 1 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
Gas phase:
  • 100 % N2

209 EUBACTERIUM LENTUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Arginine 5 28.703
Yeast extract 5 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution

212a TREPONEMA RECTALE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.778 19.556
Trehalose 1.884 5.504
Maltose x H2O 1.786 5.217
Acetic acid 1.763 29.355
Cellobiose 1.688 4.93
Sucrose 1.688 4.93
Na2CO3 1.488 14.04
Trypticase peptone 0.992 -
Ethanol 0.942 20.457
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Solutions:
  • 917 ml Solution A
  • 30 ml Solution B
  • 4 ml Solution C
  • 4 ml Solution D
  • 30 ml Solution E
  • 1 ml Solution F
  • 2 ml Solution G
  • 10 ml Solution H
  • 10 ml Solution I
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Clarified rumen fluid
  • 1 ml Vitamin K1 solution
  • 3.1 ml Volatile fatty acid mixture
  • 30 ml Sugar mix
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

228 PEPTOCOCCUS GLYCINOPHILUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Trypticase peptone 5 -
Peptone 5 -
Beef extract 5 -
Glycine 3 39.963
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
Ethanol 0.004 0.082
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution
Gas phase:
  • 80 % N2
  • 20 % CO2
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