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135a ACETOBACTERIUM MEDIUM (MARINE)

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.897 58.295
Yeast extract 1.959 -
Ethylene glycol 1.224 19.725
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
Pyridoxine hydrochloride 9.8e-5 4.8e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.1e-4
Thiamine HCl 4.9e-5 1.5e-4
Riboflavin 4.9e-5 1.3e-4
Biotin 2.0e-5 8.0e-5
Folic acid 2.0e-5 4.4e-5
Na2WO4 x 2 H2O 7.8e-6 2.4e-5
Na2SeO3 x 5 H2O 5.9e-6 2.2e-5
Vitamin B12 9.8e-7 7.2e-7
Distilled water - -
Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2