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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: Ferric ammonium citrate

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55 FRUCTOSE MINERAL MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 12 H2O 9 63.399
Fructose 5 27.753
KH2PO4 1.5 11.023
NH4Cl 1 18.695
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.01 0.068
Ferric ammonium citrate 0.005 0.019
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Solutions:
  • 500 ml Solution A
  • 300 ml Solution B
  • 200 ml Solution C
  • 0.3 ml Trace element solution SL-6

81 MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Compound Conc. [g/L] Conc. [mM]
Agar 20.101 59.764
Na2HPO4 x 2 H2O 2.915 20.531
KH2PO4 2.312 16.986
NH4Cl 1.005 18.789
MgSO4 x 7 H2O 0.503 2.039
Ferric ammonium citrate 0.05 0.192
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Solutions:
  • 5 ml Standard vitamin solution
  • 50 ml Solution A
  • 920 ml Solution B
  • 20 ml Solution C
  • 5 ml Trace element solution SL-6

81a THIOMONAS ISLANDICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 2.868 20.206
K2HPO4 2.275 13.061
NH4Cl 0.989 18.492
NaHCO3 0.495 5.887
MgSO4 x 7 H2O 0.495 2.007
Ferric ammonium citrate 0.049 -
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Solutions:
  • 5 ml Trace element solution SL-6
  • 1 ml Seven vitamins solution
Gas phase:
  • 53 % H2
  • 27 % N2
  • 20 % CO2

133 CARBON MONOXIDE OXIDIZER MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
Na2HPO4 x 12 H2O 4.5 31.699
NH4Cl 1.5 28.043
KH2PO4 0.75 5.511
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.03 0.204
Ferric ammonium citrate 0.018 0.069
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Solutions:
  • 1 ml Trace element solution SL-6

260 BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 2 H2O 4.5 31.699
Na-pyruvate 1.5 13.631
KH2PO4 1.5 11.023
NH4Cl 1 18.695
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.01 0.068
MnSO4 x H2O 0.01 0.059
Ferric ammonium citrate 0.005 -
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Solutions:
  • 3 ml Trace element solution SL-6

261 MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 2 H2O 4.5 31.699
KH2PO4 1.5 11.023
NH4Cl 1 18.695
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.01 0.068
MnSO4 x H2O 0.01 0.059
Ferric ammonium citrate 0.005 -
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Solutions:
  • 3 ml Trace element solution SL-6
Gas phase:
  • 45 % H2
  • 10 % CO2
  • 5 % O2

369 BACILLUS TUSCIAE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 2 H2O 4.5 31.699
KH2PO4 1.5 11.023
NH4Cl 1 18.695
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.01 0.068
MnSO4 x H2O 0.01 0.059
Ferric ammonium citrate 0.005 -
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Solutions:
  • 3 ml Trace element solution SL-6
Gas phase:
  • 45 % H2
  • 10 % CO2
  • 5 % O2

428 HETEROTROPHIC MEDIUM H3P

Compound Conc. [g/L] Conc. [mM]
Agar 19.802 58.877
Na2HPO4 x 2 H2O 2.871 20.226
KH2PO4 2.277 16.734
D-Glucose 1.98 10.991
Na-pyruvate 0.99 8.997
Na-lactate 0.99 8.835
D-Mannitol 0.99 5.435
NH4Cl 0.99 18.51
Na-acetate 0.99 12.069
DL-Malate 0.99 7.384
Disodium succinate 0.99 6.11
Yeast extract 0.99 -
MgSO4 x 7 H2O 0.495 2.009
Ferric ammonium citrate 0.05 -
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Solutions:
  • 50 ml Solution A
  • 855 ml Solution B
  • 20 ml Solution C
  • 30 ml Solution D
  • 50 ml Solution E
  • 5 ml Standard vitamin solution
  • 5 ml Trace element solution SL-6

442 MINERAL MEDIUM WITH TOLUENE

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 9 63.399
NH4Cl 2 37.39
KH2PO4 1.5 11.023
MgSO4 x 7 H2O 0.2 0.811
Nitrilotriacetic acid 0.01 0.052
Ferric ammonium citrate 0.005 -
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443 MINERAL MEDIUM WITH PHENYLACETATE

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 9 63.399
NH4Cl 2 37.39
KH2PO4 1.5 11.023
Phenyl acetate 0.5 3.672
MgSO4 x 7 H2O 0.2 0.811
Nitrilotriacetic acid 0.01 0.052
Ferric ammonium citrate 0.005 -
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