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Search for media using compounds and concentrations.

The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: MgCl2

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28a THIORHODOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
NaHCO3 1.5 17.856
MgCl2 x 6 H2O 1 4.919
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

28b THIORHODOCOCCUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
MgCl2 x 6 H2O 2.5 12.297
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

35 ACIDITHIOBACILLUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sulfur 10 311.818
KH2PO4 3 22.045
CaCl2 x 2 H2O 0.14 0.952
NH4Cl 0.1 1.87
MgCl2 x 6 H2O 0.1 0.492
Distilled water - -

35a THIOMONAS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		19.418 57.733
Na2S2O3 x 5 H2O 4.854 19.559
KH2PO4 2.913 21.403
Yeast extract 0.971 -
CaCl2 x 2 H2O 0.136 0.925
MgCl2 x 6 H2O 0.097 0.478
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Solutions:
  • 100 ml Solution B
  • 20 ml Solution C
  • 10 ml Solution D
  • 900 ml Soution A

79 LEUCOTHRIX MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 24 410.678
MgCl2 x 6 H2O 11 54.106
Tryptone 10 140.687
Na2SO4 4 28.161
CaCl2 x 6 H2O 2 9.129
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Solutions:
  • 1000 ml Synthetic sea water

95 DESULFUROMONAS ACETOXIDANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 19.92 340.868
Na2-DL-malate 2.649 14.88
MgCl2 x 6 H2O 1.992 9.798
NaHCO3 1.843 21.934
Na2SO4 1.763 12.412
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Solutions:
  • 951 ml Solution A
  • 3 ml Solution B
  • 40 ml Solution C
  • 10 ml Solution D
  • 1 ml Trace element solution SL-11
Gas phase:
  • 100 % N2

120d METHANOSARCINA MARINE MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 22.461 384.342
Methanol 15.469 482.765
MgCl2 x 6 H2O 9.766 48.035
Na2CO3 0.977 9.214
NH4Cl 0.488 9.128
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
  • 20 % CO2

122 ACETIVIBRIO MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellulose
optional 		10 -
Na2-ß-glycerophosphate x 5 H2O 6 19.601
K2HPO4 5.5 31.577
Cellobiose 5 14.607
Yeast extract 4.5 -
MgCl2 x 6 H2O 2.6 12.789
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Gas phase:
  • 80 % N2
  • 20 % CO2

123 MARINE AGAR

Compound Conc. [g/L] Conc. [mM]
NaCl 24 410.678
Agar 15 44.599
MgCl2 x 6 H2O 11 54.106
Bacto peptone 5 -
Na2SO4 4 28.161
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Solutions:
  • 1000 ml Synthetic sea water

124 DESULFOFARCIMEN MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.487 53.407
Na2SO4 2.832 19.935
Na-acetate 1.396 17.015
Na-butyrate 1.396 12.679
NaCl 1.167 19.961
Yeast extract 0.997 -
MgCl2 x 6 H2O 0.399 1.962
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2
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