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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

	Compound	Min. conc.	Max. conc.	Unit

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Current criteria: HEPES

Your search received 76 results.

Compound	Conc. [g/L]	Conc. [mM]
HEPES	11.79	49.477
2-Furoic acid	2.216	19.768
KHCO₃	1.978	19.76
NH₄Cl	0.989	18.492
KH₂PO₄	0.297	2.181
L-Cysteine HCl x H₂O	0.297	1.689
Na₂S x 9 H₂O	0.297	1.235
Nitrilotriacetic acid	0.127	0.662
MgCl₂ x 6 H₂O	0.099	0.487
NaCl	0.01	0.169
FeCl₂ x 4 H₂O	0.01	0.05
NiCl₂ x 6 H₂O	9.9e-4	0.008
ZnCl₂	9.9e-4	0.007
CaCl₂ x 2 H₂O	9.9e-4	0.007
MnCl₂ x 4 H₂O	9.9e-4	0.005
Sodium resazurin	4.9e-4	0.002
Na₂WO₄ x 2 H₂O	4.0e-4	0.001
CoCl₂ x 6 H₂O	3.0e-4	0.002
Na₂MoO₄ x 2 H₂O	3.0e-4	0.001
Na₂SeO₃ x 5 H₂O	3.0e-4	0.001
CuCl₂	2.0e-4	0.001
H₃BO₃	9.9e-5	0.002
Pyridoxine hydrochloride	9.9e-5	4.8e-4
Nicotinic acid	4.9e-5	4.0e-4
p-Aminobenzoic acid	4.9e-5	3.6e-4
(DL)-alpha-Lipoic acid	4.9e-5	2.4e-4
Calcium D-(+)-pantothenate	4.9e-5	2.1e-4
Thiamine HCl	4.9e-5	1.5e-4
Riboflavin	4.9e-5	1.3e-4
Biotin	2.0e-5	8.1e-5
Folic acid	2.0e-5	4.5e-5
Vitamin B₁₂	9.9e-7	7.3e-7
Distilled water	-	-
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Solutions:

10 ml Trace element solution
1 ml Wolin's vitamin solution (10x)

Gas phase:

80 % N2
20 % CO2

Compound	Conc. [g/L]	Conc. [mM]
Bovine serum albumin	35.025	-
Gelatine	9.797	-
HEPES buffer	4.199	17.62
Proteose peptone no. 2	3.499	-
Glucose	2.099	11.653
Proteose yeastolate	0.7	-
Tryptone peptone	0.7	-
Na-pyruvate	0.56	5.087
Sodium citrate	0.49	1.898
N-Acetylglucosamine	0.28	1.265
MgCl₂ x 6 H₂O	0.21	1.033
L-Glutamine	0.07	0.479
Rabbit serum	-	31675
Double distilled water	-	-
CMRL 1066	-	-
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Solutions:

1000 ml Solution A
200 ml Solution B
143 ml Solution C
86 ml Solution D

Compound	Conc. [g/L]	Conc. [mM]
MgCl₂ x 6 H₂O	6	29.513
HEPES	5	20.982
Trypticase peptone	2	-
Yeast extract	2	-
Trimethylamine-HCl	2	-
KCl	1.5	20.121
NH₄Cl	1	18.695
K₂HPO₄ x 3 H₂O	0.4	2.297
Na₂S x 9 H₂O	0.25	1.041
2-Mercaptoethanesulfonic acid	0.2	1.406
MgSO₄ x 7 H₂O	0.03	0.122
Nitrilotriacetic acid	0.015	0.078
NaCl	0.01	0.171
MnSO₄ x H₂O	0.005	0.03
CoSO₄ x 7 H₂O	0.002	0.012
ZnSO₄ x 7 H₂O	0.002	0.006
CaCl₂ x 2 H₂O	1.0e-3	0.007
FeSO₄ x 7 H₂O	1.0e-3	0.004
Sodium resazurin	5.0e-4	-
NiCl₂ x 6 H₂O	3.0e-4	0.002
AlK(SO₄)₂ x 12 H₂O	2.0e-4	4.2e-4
H₃BO₃	1.0e-4	0.002
Na₂MoO₄ x 2 H₂O	1.0e-4	4.1e-4
CuSO₄ x 5 H₂O	1.0e-4	4.0e-4
Na₂WO₄ x 2 H₂O	4.0e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
Distilled water	-	-
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Solutions:

10 ml Modified Wolin's mineral solution

Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
HEPES	7.992	33.538
Na-acetate	1.641	20
K₂HPO₄	0.999	5.736
Yeast extract	0.999	-
NH₄Cl	0.5	9.338
Peptone	0.5	-
Biotin	0.1	0.409
MgSO₄ x 7 H₂O	0.003	0.012
Nitrilotriacetic acid	0.001	0.008
NaCl	1.0e-3	0.017
MnSO₄ x H₂O	5.0e-4	0.003
CoSO₄ x 7 H₂O	1.8e-4	0.001
ZnSO₄ x 7 H₂O	1.8e-4	6.3e-4
CaCl₂ x 2 H₂O	1.0e-4	6.8e-4
FeSO₄ x 7 H₂O	1.0e-4	3.6e-4
NiCl₂ x 6 H₂O	3.0e-5	2.3e-4
AlK(SO₄)₂ x 12 H₂O	2.0e-5	4.2e-5
H₃BO₃	1.0e-5	1.6e-4
Na₂MoO₄ x 2 H₂O	1.0e-5	4.1e-5
CuSO₄ x 5 H₂O	1.0e-5	4.0e-5
Na₂WO₄ x 2 H₂O	4.0e-7	1.2e-6
Na₂SeO₃ x 5 H₂O	3.0e-7	1.1e-6
Distilled water	-	-
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Solutions:

1 ml Modified Wolin's mineral solution

Compound	Conc. [g/L]	Conc. [mM]
HEPES	5.2	21.821
Agar	2	5.947
KH₂PO₄	0.015	0.112
Na₂CO₃	0.01	0.094
Ferric ammonium citrate	0.003	-
H₃BO₃	0.003	0.046
MnCl₂ x 4 H₂O	0.002	0.009
Na₂-EDTA	5.0e-4	0.001
Na₂MoO₄ x 2 H₂O	3.9e-4	0.002
ZnSO₄ x 7 H₂O	2.2e-4	7.7e-4
CuSO₄ x 5 H₂O	7.9e-5	3.2e-4
Distilled water	-	-
Sea water	-	-
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Solutions:

1 ml Trace elements (A5 of Rippka et al., 1979)

Compound	Conc. [g/L]	Conc. [mM]
HEPES	2.38	9.987
Sucrose	2	5.843
Tryptone	1	14.069
KNO₃	1	9.891
NH₄Cl	0.33	6.169
KCl	0.33	4.427
MgCl₂ x 6 H₂O	0.33	1.623
Yeast extract	0.2	-
MgSO₄ x 7 H₂O	0.003	0.012
Nitrilotriacetic acid	0.002	0.008
NaCl	1.0e-3	0.017
MnSO₄ x H₂O	5.0e-4	0.003
CoSO₄ x 7 H₂O	1.8e-4	0.001
ZnSO₄ x 7 H₂O	1.8e-4	6.3e-4
CaCl₂ x 2 H₂O	1.0e-4	6.8e-4
FeSO₄ x 7 H₂O	1.0e-4	3.6e-4
NiCl₂ x 6 H₂O	3.0e-5	2.3e-4
AlK(SO₄)₂ x 12 H₂O	2.0e-5	4.2e-5
H₃BO₃	1.0e-5	1.6e-4
Pyridoxine hydrochloride	1.0e-5	4.9e-5
Na₂MoO₄ x 2 H₂O	1.0e-5	4.1e-5
CuSO₄ x 5 H₂O	1.0e-5	4.0e-5
Nicotinic acid	5.0e-6	4.1e-5
p-Aminobenzoic acid	5.0e-6	3.6e-5
(DL)-alpha-Lipoic acid	5.0e-6	2.4e-5
Thiamine HCl	5.0e-6	1.5e-5
Riboflavin	5.0e-6	1.3e-5
Calcium D-(+)-pantothenate	5.0e-6	-
Biotin	2.0e-6	8.2e-6
Folic acid	2.0e-6	4.5e-6
Na₂WO₄ x 2 H₂O	4.0e-7	1.2e-6
Na₂SeO₃ x 5 H₂O	3.0e-7	1.1e-6
Vitamin B₁₂	1.0e-7	7.4e-8
Distilled water	-	-
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Solutions:

1 ml Wolin's vitamin solution
1 ml Modified Wolin's mineral solution

Compound	Conc. [g/L]	Conc. [mM]
HEPES buffer	6	25.178
MgCl₂ x 6 H₂O	0.6	2.951
CaCl₂ x 2 H₂O	0.3	2.041
Distilled water	-	-

Compound	Conc. [g/L]	Conc. [mM]
Agar	20	59.465
NaCl	10	171.116
HEPES	2	8.393
MgSO₄ x 7 H₂O	1.6	6.492
Na-caseinate	0.5	-
CaCl₂ x 2 H₂O	0.2	1.36
NaHCO₃	0.16	1.905
KCl	0.1	1.341
KBr	0.08	0.672
SrCl2 x 6 H₂O	0.03	0.113
Fe(III) citrate	0.02	0.082
Na₂-EDTA	1.0e-3	0.003
H₃BO₃	6.0e-4	0.01
Vitamin B₁₂	5.0e-4	3.7e-4
CoCl₂ x 6 H₂O	4.0e-4	0.003
FeSO₄ x 7 H₂O	4.0e-4	0.001
ZnSO₄ x 7 H₂O	2.0e-4	7.0e-4
MnCl₂ x 4 H₂O	6.0e-5	3.0e-4
Na₂MoO₄ x 2 H₂O	6.0e-5	2.5e-4
NiCl₂ x 6 H₂O	4.0e-5	3.1e-4
CuCl₂ x 2 H₂O	2.0e-5	1.2e-4
Distilled water	-	-
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Solutions:

2 ml Trace element solution SL-4

Compound	Conc. [g/L]	Conc. [mM]
Sea Salt	34.965	-
HEPES	2.498	10.481
Na-pyruvate	1.099	9.986
Yeast extract	0.999	-
NH₄Cl	0.1	1.868
KH₂PO₄	0.05	0.367
L-Histidine	0.04	0.258
L-Cysteine HCl x H₂O	0.04	0.228
MgSO₄ x 7 H₂O	0.03	0.122
NaCl	0.01	0.171
MnSO₄ x H₂O	0.005	0.03
(NH₄)₂Ni(SO₄)₂ x 6 H₂O	0.003	0.007
CoCl₂ x 6 H₂O	0.002	0.014
ZnSO₄ x 7 H₂O	0.002	0.006
CaCl₂ x 2 H₂O	1.0e-3	0.007
FeSO₄ x 7 H₂O	1.0e-3	0.004
AlK(SO₄)₂ x 12 H₂O	1.8e-4	3.8e-4
Pyridoxine hydrochloride	1.5e-4	7.3e-4
H₃BO₃	1.0e-4	0.002
Nicotinic acid	1.0e-4	8.1e-4
Na₂SeO₄	1.0e-4	5.3e-4
Na₂MoO₄ x 2 H₂O	1.0e-4	4.1e-4
CuSO₄ x 5 H₂O	1.0e-4	4.0e-4
Na₂WO₄ x 2 H₂O	1.0e-4	3.0e-4
Thiamine-HCl x 2 H₂O	1.0e-4	2.7e-4
Calcium pantothenate	5.0e-5	1.0e-4
Vitamin B₁₂	5.0e-5	3.7e-5
p-Aminobenzoic acid	4.0e-5	2.9e-4
D-(+)-biotin	1.0e-5	4.1e-5
Distilled water	-	-
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Solutions:

1 ml Wolfe's mineral elixir
0.5 ml Seven vitamins solution

Compound	Conc. [g/L]	Conc. [mM]
NaCl	180	3080.08
MgCl₂ x 6 H₂O	20	98.375
HEPES	6	25.178
K₂SO₄	5	28.693
NH₄Cl	0.5	9.348
Sucrose	0.5	1.461
CaCl₂ x 2 H₂O	0.1	0.68
Yeast extract	0.1	-
KH₂PO₄	0.05	0.367
Distilled water	-	-
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Show 1 to 10 (76 total)

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