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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Hemin

Your search received 27 results.

78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
Vitamin K3 0.047 -
Hemin 0.005 0.007
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
Casamino acids 0.16 -
Yeast extract 0.16 -
Tryptone peptone 0.16 -
Ascorbic acid 0.1 0.568
L-Glutamine 0.07 0.479
L-Histidin 0.06 0.387
L-Serin 0.05 0.476
Vitamin K3 0.05 -
Resazurin 1.0e-3 0.004
Hemin 5.0e-4 7.7e-4
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

104 PYG MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
L-Cysteine HCl x H2O 0.5 -
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution

110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
D-Glucose 4 22.203
Maltose 1 2.921
Cellobiose 1 2.921
Starch 1 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
Gas phase:
  • 100 % N2

209 EUBACTERIUM LENTUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Arginine 5 28.703
Yeast extract 5 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution

228 PEPTOCOCCUS GLYCINOPHILUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Trypticase peptone 5 -
Peptone 5 -
Beef extract 5 -
Glycine 3 39.963
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution
Gas phase:
  • 80 % N2
  • 20 % CO2

330 RUMEN BACTERIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 4 37.74
Trypticase peptone 2 -
Glycerol 0.5 5.429
D-Glucose 0.5 2.775
Maltose 0.5 1.461
Cellobiose 0.5 1.461
Starch 0.5 -
Yeast extract 0.5 -
NaCl 0.456 7.803
K2HPO4 0.3 1.722
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
(NH4)2SO4 0.228 1.725
KH2PO4 0.228 1.675
MgSO4 x 7 H2O 0.095 0.385
CaCl2 x 2 H2O 0.061 0.414
Hemin 1.0e-3 0.002
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Solutions:
  • 38 ml Mineral solution
  • 2 ml Haemin solution
  • 3.1 ml Volatile fatty acid mixture
Gas phase:
  • 100 % CO2

413 BTU MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
Na-formiate 1.8 26.468
Na-fumarate 1.8 11.248
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 30 ml Formiate/Fumarate Solution

523 CLOSTRIDIUM CHARTATABIDUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Na2CO3 4 37.74
Trypticase peptone 2 -
Yeast extract 0.5 -
NaCl 0.456 7.803
K2HPO4 0.3 1.722
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
(NH4)2SO4 0.228 1.725
KH2PO4 0.228 1.675
MgSO4 x 7 H2O 0.095 0.385
CaCl2 x 2 H2O 0.061 0.414
Hemin 1.0e-3 0.002
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Solutions:
  • 38 ml Mineral solution
  • 200 ml Clarified rumen fluid
  • 2 ml Haemin solution
Gas phase:
  • 100 % CO2

751 SC MEDIUM

Compound Conc. [g/L] Conc. [mM]
Corn meal agar 17 -
Soy peptone 8 -
Bovine serum albumin 2 -
Cysteine 1 8.254
KH2PO4 1 7.348
K2HPO4 1 5.741
Glucose 0.5 2.775
MgSO4 x 7 H2O 0.2 0.811
Hemin 0.015 0.023
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