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78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
Haemin 0.003 0.004
Sodium resazurin 5.9e-4 0.002
Pyridoxine hydrochloride 1.2e-4 5.7e-4
Nicotinic acid 5.9e-5 4.8e-4
p-Aminobenzoic acid 5.9e-5 4.3e-4
(DL)-alpha-Lipoic acid 5.9e-5 2.8e-4
Thiamine HCl 5.9e-5 1.7e-4
Riboflavin 5.9e-5 1.6e-4
Calcium D-(+)-pantothenate 5.9e-5 -
Biotin 2.3e-5 9.6e-5
Folic acid 2.3e-5 5.3e-5
Vitamin B12 1.2e-6 8.6e-7
NaOH - -
Vitamin K1 - -
Horse serum - -
Isobutyric acid - -
DL-2-Methylbutyric acid - -
Valeric acid - -
Isovaleric acid - -
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

119 METHANOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.399 7.456
NaCl 0.399 6.824
MgSO4 x 7 H2O 0.399 1.618
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Isobutyric acid - -
DL-2-Methylbutyric acid - -
Valeric acid - -
Isovaleric acid - -
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Solutions:
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

119a METHANOBREVIBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
Yeast extract 0.997 -
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.399 7.456
NaCl 0.399 6.824
MgSO4 x 7 H2O 0.399 1.618
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Distilled water - -
H2SO4 - -
DL-2-Methylbutyric acid - -
Valeric acid - -
Isovaleric acid - -
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 200 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 80 % H2
  • 20 % CO2

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
L-Cysteine HCl x H2O 0.498 2.835
Na2S x 9 H2O 0.498 2.073
NH4Cl 0.398 7.448
NaCl 0.398 6.817
MgSO4 x 7 H2O 0.398 1.616
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
H2SO4 - -
DL-2-Methylbutyric acid - -
Isovaleric acid - -
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

119c GUDONGELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 2 29.409
Na-acetate 1 12.19
NaHCO3 0.5 5.952
KH2PO4 0.5 3.674
L-Cysteine HCl x H2O 0.5 2.847
Na2S x 9 H2O 0.5 2.082
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
Yeast extract 0.2 -
CaCl2 x 2 H2O 0.05 0.34
HCl 0.003 0.069
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.002 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Valeric acid - -
DL-2-Methylbutyric acid - -
Isobutyric acid - -
Sludge - -
Distilled water - -
Isovaleric acid - -
Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 100 % N2

161 METHANOFOLLIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.998 23.784
Yeast extract 0.999 -
Trypticase 0.999 -
L-Cysteine HCl x H2O 0.5 2.844
Na2S x 9 H2O 0.5 2.08
(NH4)2SO4 0.3 2.268
KH2PO4 0.3 2.202
K2HPO4 0.3 1.721
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
FeSO4 x 7 H2O 0.002 0.007
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
Sodium resazurin 5.0e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Nicotinic acid 5.0e-5 4.1e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
Vitamin B12 1.0e-6 7.4e-7
Isovaleric acid - -
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Solutions:
  • 300 ml Clarified rumen fluid
  • 10 ml Modified Wolin's mineral solution
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

436 RUMINOCOCCUS ALBUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Tryptone 5 70.343
Na2CO3 4 37.74
Glucose 3 16.652
Cellobiose 2 5.843
Yeast extract 2 -
(NH4)2SO4 0.8 6.054
L-Cysteine HCl x H2O 0.5 2.847
NaCl 0.48 8.214
KH2PO4 0.24 1.764
K2HPO4 0.24 1.378
MgSO4 x 7 H2O 0.1 0.406
CaCl2 x 7 H2O 0.064 0.577
Resazurin 1.0e-3 0.004
Distilled water - -
Isovaleric acid - -
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Solutions:
  • 40 ml Mineral solution 1
  • 40 ml Mineral solution 2
  • 1 ml Fatty acid mixture

719 ANAEROFILUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Glucose 5.241 29.091
NaHCO3 4.193 49.911
Na-formate 2.096 30.827
Na-acetate 1.048 12.778
Yeast extract 1.048 -
KH2PO4 0.524 3.851
L-Cysteine HCl x H2O 0.524 2.984
Na2S x 9 H2O 0.524 2.182
NH4Cl 0.419 7.839
NaCl 0.419 7.175
MgSO4 x 7 H2O 0.419 1.701
CaCl2 x 2 H2O 0.052 0.357
HCl 0.003 0.072
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.002 0.008
Sodium resazurin 5.0e-4 -
CoCl2 x 6 H2O 2.0e-4 0.002
MnCl2 x 4 H2O 1.0e-4 5.3e-4
ZnCl2 7.3e-5 5.4e-4
Na2MoO4 x 2 H2O 3.8e-5 1.6e-4
NiCl2 x 6 H2O 2.5e-5 1.9e-4
H3BO3 6.3e-6 1.0e-4
CuCl2 x 2 H2O 2.1e-6 1.2e-5
Valeric acid - -
Isovaleric acid - -
Show more
Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 20 ml Fatty acid mixture
Gas phase:
  • 80 % N2
  • 20 % CO2

829 DESULFUROBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sea Salt 29.851 -
Sulfur 9.95 310.267
MES [2-(N-morpholino) ethane sulfonic acid] 1.94 9.938
NH4Cl 0.995 18.602
Na2CO3 0.498 4.694
KH2PO4 0.348 2.559
NaHCO3 0.05 0.592
Na2S2O4 0.05 0.286
Succinic acid 0.006 0.051
Isobutyric acid 0.005 0.056
2-Methylbutyric acid 0.005 0.049
Valeric acid 0.005 0.049
3-Methylbutyric acid 0.005 0.049
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
  • 1 ml Growth-stimulating factors
  • 1 ml Na-dithionite solution (5% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

829b THERMODESULFOBACTERIUM HYDROGENIPHILUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sea Salt 29.851 -
MES [2-(N-morpholino) ethane sulfonic acid] 1.94 9.938
NH4Cl 0.995 18.602
Na2CO3 0.498 4.694
Yeast extract 0.498 -
KH2PO4 0.348 2.559
NaHCO3 0.05 0.592
Na2S2O4 0.05 0.286
Succinic acid 0.006 0.051
Isobutyric acid 0.005 0.056
2-Methylbutyric acid 0.005 0.049
Valeric acid 0.005 0.049
3-Methylbutyric acid 0.005 0.049
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
  • 1 ml Growth-stimulating factors
  • 1 ml Na-dithionite solution (5% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2
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