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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: K2HPO4 x 3 H2O

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137 MINERAL LACTATE MEDIUM (low carbon content)

Compound Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 1.13 6.488
NH4Cl 1 18.695
NaCl 1 17.112
KH2PO4 0.88 6.467
Na-lactate 0.5 4.462
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Solutions:
  • 1.2 ml Trace element solution (Vishniac & Santer, 1957)

150 ACIDIANUS BRIERLEYI MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sulfur 10 311.818
(NH4)2SO4 3 22.703
K2HPO4 x 3 H2O 0.5 2.871
MgSO4 x 7 H2O 0.5 2.029
Yeast extract 0.2 -
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150a ACIDITHIOBACILLUS CALDUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sulfur 4.951 154.365
(NH4)2SO4 2.97 22.478
K2HPO4 x 3 H2O 0.495 2.842
MgSO4 x 7 H2O 0.495 2.009
KCl 0.099 1.328
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Solutions:
  • 10 ml Trace element solution

255 CLOSTRIDIUM (GS) MEDIUM

Compound Conc. [g/L] Conc. [mM]
Morpholinopropane sulfonic acid 9.98 47.692
Yeast extract 5.988 -
Cellobiose 4.99 14.578
Urea 1.996 33.236
K2HPO4 x 3 H2O 0.998 5.73
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Gas phase:
  • 100 % N2

320 CLOSTRIDIUM CELLULOVORANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
NH4Cl 1 18.695
Na2CO3 1 9.435
K2HPO4 x 3 H2O 1 5.741
KCl 0.5 6.707
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Solutions:
  • 20 ml Sludge fluid
  • 1 ml Trace element solution SL-10
  • 20 ml Clarified rumen fluid
Gas phase:
  • 80 % N2
  • 20 % CO2

321 METHANOCORPUSCULUM AGGREGANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 5 73.522
Na2CO3 1.5 14.153
NH4Cl 1 18.695
Na-acetate 1 12.19
Trypticase peptone 1 -
K2HPO4 x 3 H2O 0.4 2.297
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Solutions:
  • 50 ml Minerals solution
  • 10 ml Modified Wolin's mineral solution
  • 5 ml Clarified rumen fluid
  • 5 ml Sludge fluid
Gas phase:
  • 80 % H2
  • 20 % CO2

332 METHANOCULLEUS BOURGENSIS MEDIUM

Gas phase:
  • 80 % H2
  • 20 % CO2

348 METHANOHALOPHILUS PORTUCALENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 120 2053.39
MgCl2 x 6 H2O 7 34.431
MgSO4 x 7 H2O 6 24.343
KCl 3.8 50.973
Yeast extract 2 -
Trypticase peptone 2 -
Trimethylamine-HCl 1.9 -
Na2CO3 1.5 14.153
NH4Cl 1 18.695
CaCl2 x 2 H2O 0.5 3.401
L-Cysteine HCl x H2O 0.5 2.847
K2HPO4 x 3 H2O 0.4 2.297
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
Gas phase:
  • 80 % N2
  • 20 % CO2

408 PSYCHRILYOBACTER SP. MEDIUM

Compound Conc. [g/L] Conc. [mM]
L-Glutamic acid 20 135.934
Peptone 10 -
Casamino acids 10 -
Yeast extract 3 -
NH4Cl 2 37.39
K2HPO4 x 3 H2O 2 11.483
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Solutions:
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 100 % N2

479 METHANOHALOPHILUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 6 29.513
Trypticase peptone 2 -
Yeast extract 2 -
Trimethylamine-HCl 2 -
KCl 1.5 20.121
Na2CO3 1.5 14.153
NH4Cl 1 18.695
K2HPO4 x 3 H2O 0.4 2.297
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Solutions:
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % N2
  • 20 % CO2
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