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Search for media using compounds and concentrations.

The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: L-Cysteine HCl x H2O

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11b MRS MEDIUM (pre-reduced)

Compound Conc. [g/L] Conc. [mM]
Glucose 20 111.012
Casein peptone 10 -
Meat extract 10 -
Na-acetate 5 60.951
Yeast extract 5 -
K2HPO4 2 11.483
(NH4)3 citrate 2 8.223
Tween 80 1 0.764
L-Cysteine HCl x H2O 0.5 2.847
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52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
L-Cysteine HCl x H2O 0.25 1.423
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

58 BIFIDOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glucose 10 55.506
Casein peptone 10 -
Bacto Soytone 5 -
Meat extract 5 -
Yeast extract 5 -
L-Cysteine HCl x H2O 0.5 -
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Solutions:
  • 40 ml Salt solution

59 LEUCONOSTOC OENOS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glucose 9.99 55.451
Casein peptone 9.99 -
Fructose 4.995 27.725
Yeast extract 4.995 -
(NH4) citrate 3.497 -
L-Cysteine HCl x H2O 0.5 -
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60 MOORELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glucose 17.946 99.612
K2HPO4 6.979 40.069
Yeast extract 4.985 -
Tryptone 4.985 -
KH2PO4 4.487 32.969
Na2CO3 1.994 18.814
(NH4)2SO4 0.499 3.773
L-Cysteine HCl x H2O 0.299 1.703
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Solutions:
  • 843 ml Solution A
  • 50 ml Solution B
  • 50 ml Solution C
  • 40 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
Gas phase:
  • 100 % CO2

78b CHOPPED MEAT MEDIUM (N2/CO2)

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
K2HPO4 5 28.707
Yeast extract 5 -
L-Cysteine HCl x H2O 0.5 2.847
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K1 solution
  • 1 ml Vitamin K3 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

104 PYG MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
L-Cysteine HCl x H2O 0.5 -
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution

104a ANAEROBACULUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Trypticase peptone 5 -
Meat peptone 5 -
Na2S2O3 x 5 H2O 2.5 10.073
D-Glucose 1 5.551
L-Cysteine HCl x H2O 0.5 2.847
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Solutions:
  • 40 ml Salt solution
Gas phase:
  • 100 % N2

104b PY + X MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
D-Glucose 5 27.753
Trypticase peptone 5 -
Meat peptone 5 -
L-Cysteine HCl x H2O 0.5 2.847
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Solutions:
  • 40 ml Salt solution
Gas phase:
  • 100 % N2
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