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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

	Compound	Min. conc.	Max. conc.	Unit

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Current criteria: L-Glutamic acid

Your search received 11 results.

Compound	Conc. [g/L]	Conc. [mM]
MgCl₂ x 6 H₂O	9.872	48.556
L-Glutamic acid	3.949	26.838
CaCl₂ x 2 H₂O	3.653	24.845
Casamino acids	1.974	-
Nutrient broth	1.974	-
Yeast extract	1.974	-
KCl	0.74	9.931
L-Cysteine HCl x H₂O	0.494	2.81
MgSO₄ x 7 H₂O	0.03	0.12
Nitrilotriacetic acid	0.015	0.077
NaCl	0.01	0.169
MnSO₄ x H₂O	0.005	0.029
FeSO₄ x 7 H₂O	0.002	0.007
CoSO₄ x 7 H₂O	0.002	0.011
ZnSO₄ x 7 H₂O	0.002	0.006
Sodium resazurin	4.9e-4	0.002
NiCl₂ x 6 H₂O	3.0e-4	0.002
AlK(SO₄)₂ x 12 H₂O	2.0e-4	4.2e-4
H₃BO₃	9.9e-5	0.002
Pyridoxine hydrochloride	9.9e-5	4.8e-4
Na₂MoO₄ x 2 H₂O	9.9e-5	4.1e-4
CuSO₄ x 5 H₂O	9.9e-5	4.0e-4
Nicotinic acid	4.9e-5	4.0e-4
p-Aminobenzoic acid	4.9e-5	3.6e-4
(DL)-alpha-Lipoic acid	4.9e-5	2.4e-4
Calcium D-(+)-pantothenate	4.9e-5	2.1e-4
Thiamine HCl	4.9e-5	1.5e-4
Riboflavin	4.9e-5	1.3e-4
Biotin	2.0e-5	8.1e-5
Folic acid	2.0e-5	4.5e-5
Na₂WO₄ x 2 H₂O	3.9e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
Vitamin B₁₂	9.9e-7	7.3e-7
Distilled water	-	-
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Solutions:

10 ml Modified Wolin's mineral solution
1 ml Wolin's vitamin solution (10x)

Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
L-Glutamic acid	20	135.934
Peptone	10	-
Casamino acids	10	-
Yeast extract	3	-
NH₄Cl	2	37.39
K₂HPO₄ x 3 H₂O	2	11.483
L-Cysteine HCl x H₂O	0.5	2.847
MgSO₄ x 7 H₂O	0.03	0.122
Nitrilotriacetic acid	0.015	0.078
NaCl	0.01	0.171
MnSO₄ x H₂O	0.005	0.03
CoSO₄ x 7 H₂O	0.002	0.012
ZnSO₄ x 7 H₂O	0.002	0.006
CaCl₂ x 2 H₂O	1.0e-3	0.007
FeSO₄ x 7 H₂O	1.0e-3	0.004
Sodium resazurin	5.0e-4	-
NiCl₂ x 6 H₂O	3.0e-4	0.002
AlK(SO₄)₂ x 12 H₂O	2.0e-4	4.2e-4
H₃BO₃	1.0e-4	0.002
Na₂MoO₄ x 2 H₂O	1.0e-4	4.1e-4
CuSO₄ x 5 H₂O	1.0e-4	4.0e-4
Na₂WO₄ x 2 H₂O	4.0e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
Distilled water	-	-
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Solutions:

10 ml Modified Wolin's mineral solution

Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
Bovine albumin	5	-
Disodium Phosphate	2.5	17.611
Dextrose	2	10.092
Monopotassium Phosphate	1	7.348
Ammonium Sulfate	0.5	3.784
L-Glutamic acid	0.5	3.398
Sodium citrate	0.1	0.387
Magnesium sulfate	0.05	0.415
Ferric ammonium citrate	0.04	-
Copper Sulfate	1.0e-3	0.006
Zinc Sulfate	1.0e-3	0.006
Pyridoxine	1.0e-3	0.006
Calcium Chloride	5.0e-4	0.003
Biotin	5.0e-4	0.002
Catalase	3.0e-4	-
Distilled water	-	-
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Solutions:

100 ml ADC Enrichment

Compound	Conc. [g/L]	Conc. [mM]
Bovine albumin	5	-
Dextrose	2	10.092
NaCl	0.85	14.545
Agar	0.35	1.041
Oleic acid	0.05	0.177
KH₂PO₄	0.035	0.257
Na₂HPO₄	0.035	0.247
Pancreatic digest of casein	0.023	-
(NH₄)₂SO₄	0.012	0.088
L-Glutamic acid	0.012	0.079
Sodium citrate	0.009	0.036
Catalase	0.004	-
MgSO₄	0.001	0.01
Ferric ammonium citrate	9.3e-4	-
Pyridoxine	2.3e-5	1.4e-4
Malachite green	2.3e-5	6.4e-5
Biotin	1.2e-5	4.8e-5
Glycerol	-	-
Distilled water	-	-
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Solutions:

21 ml Bacto Middelbrook 7H11 agar (Difco 283810)
100 ml Bacto Middelbrook OADC enrichment (Difco 212240):

Compound	Conc. [g/L]	Conc. [mM]
K₂HPO₄	14	80.379
Glucose	10	55.506
KH₂PO₄	6	44.09
(NH₄)₂SO₄	2	15.136
Trisodium citrate x 2 H₂O	1	3.4
DL-Asparagine x H₂O	0.8	6.055
Glycine	0.2	2.664
L-Proline	0.2	1.737
L-Glutamic acid	0.2	1.359
L-Histidine	0.2	1.289
L-Tryptophan	0.2	0.979
MgSO₄ x 7 H₂O	0.2	0.811
L-Arginine x HCl	0.2	-
Vitamin B₁₂	4.0e-5	3.0e-5
Distilled water	-	-
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Compound	Conc. [g/L]	Conc. [mM]
NaCl	249.377	4267.22
Agar	24.938	74.146
K₂SO₄	6.234	35.777
Casamino acids	4.988	-
L-Glutamic acid	2.494	16.949
Trisodium citrate x 2 H₂O	2.494	8.479
Yeast extract	2.494	-
NH₄Cl	1.247	23.311
KH₂PO₄	1.247	9.163
FeSO₄ x 6 H₂O	0.005	0.033
Na₂S₂O₃ x 5 H₂O	0.005	0.02
CaCl₂ x 2 H₂O	0.002	0.017
CoCl₂ x 6 H₂O	7.5e-4	0.006
NiCl₂ x 6 H₂O	2.5e-4	0.002
ZnCl₂	2.5e-4	0.002
MnCl₂ x 4 H₂O	2.5e-4	0.001
Na₂MoO₄ x 2 H₂O	2.5e-4	0.001
AlCl3	1.0e-4	7.5e-4
Na₂WO₄ x 2 H₂O	5.0e-5	1.5e-4
H₃BO₄	5.0e-5	-
CuCl₂ x 6 H₂O	2.5e-5	1.9e-4
BaCl2 x 2 H₂O	2.5e-5	1.0e-4
Distilled water	-	-
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Solutions:

2 ml Trace Element Solution

Compound	Conc. [g/L]	Conc. [mM]
(NH₄)₆Mo₇O₂₄ x 4 H₂O	12.4	-
Yeast extract	3	-
Fe SO₄ x 7 H₂O	2.78	10
D-Arabinose	2	13.322
L-Rhamnose	2	12.183
L-Fucose	2	12.183
D-Mannose	2	11.102
D-Glucose	2	11.101
D-Trehalose	2	5.843
D-Sucrose	2	5.843
NH₄Cl	1.6	29.912
Glutathione	1.537	5
Ethanol	1	21.706
NaHCO₃	1	11.904
Ascorbic acid	1	5.678
KCl	0.968	12.985
D-Fructose	0.8	4.441
D-Mannitol	0.8	4.392
D-glucuronic acid	0.8	4.121
D-galacturonic acid	0.8	4.121
D-Maltose	0.8	2.337
L-Glutamine	0.68	4.653
L-Histidine	0.62	3.996
NaVO₃	0.61	5.003
D	0.56	4.866
Pyruvic acid	0.55	6.246
L-Serine	0.525	4.996
Fumaric acid	0.5	4.308
HCl	0.365	10
L-Cysteine HCl	0.35	2.221
Formic acid	0.3	6.518
L-Glutamic acid	0.3	2.039
ZnSO₄ x 7 H₂O	0.287	0.998
MgSO₄ x 7 H₂O	0.25	1.014
DL-carnitine	0.2	1.241
Citric acid	0.2	1.041
N-Acetylglucosamine	0.2	0.904
L-Lysine HCl	0.182	-
L-Arginine	0.175	1.005
L-Ornithine HCl	0.17	-
L-Phenylalanine	0.165	0.999
CaCl₂ x H₂O	0.15	1.352
L-Asparagine	0.15	1.135
L-Methionine	0.15	1.005
NaH₂PO₄ x H₂O	0.14	1.167
L-Isoleucine	0.131	0.999
L-Leucine	0.131	0.999
NiSO₄ x 6 H₂O	0.131	0.846
L-Threonine	0.12	1.007
L-Aspartic acid	0.118	0.887
SnCl₂ x 2 H₂O	0.118	0.523
L-Valine	0.117	0.999
L-Proline	0.115	0.999
L-Tryptophan	0.102	0.499
Nicotinic acid	0.1	0.812
Folinic acid	0.1	0.211
L-Tyrosine	0.09	0.497
NaOH	0.08	2
Glycine	0.075	0.999
Nicotinamide	0.05	0.409
Choline chloride	0.05	0.358
Myo-inositol	0.05	0.278
D-(+)-biotin	0.05	0.205
Folic acid	0.05	0.113
Vitamine B₁₂	0.05	0.037
L-Alanine	0.045	0.505
Hemin	0.026	0.04
N-acetylmuramic acid	0.025	0.085
Na₂SeO₃	0.017	0.1
MnSO₄ x H₂O	0.017	0.1
2-Mercaptoethanesulfonic acid	0.01	0.07
Phenol red	0.01	0.028
Riboflavine	0.01	0.027
Pyridoxal hydrochloride	0.005	0.025
Pyridoxal phosphate	0.005	0.02
Thiamine HCl	0.005	0.015
Thiamin pyrophosphate	0.005	0.012
Calcium D-(+)-pantothenate	0.005	-
Putrescine 2 HCl	0.005	-
Thymidine	0.002	0.01
N-(2-acetamido)-2-aminoethanesulfonic acid	0.002	0.01
Hypoxanthine	0.001	0.01
Uracil	0.001	0.01
Coenzym A	1.0e-3	0.001
Flavin adenine dinucleotide	1.0e-3	0.001
ß-nicotinamide adenine dinucleotide	1.0e-3	-
CuSO₄	7.9e-4	0.005
Cholesterol	-	-
Asialofetuin	-	-
DL-alpha-lipoic acid	-	-
2-Mercaptoethanol	-	-
Isovaleric acid	-	-
Valeric acid	-	-
2-Methylbutyric acid	-	-
Isobutyric acid	-	-
Distilled water	-	-
Rabbit serum	-	-
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Solutions:

200 ml Solution H
8 ml Solution I
2 ml Solution J
40 ml Solution K
2 ml Solution L
200 ml Trace elements III
10 ml Solution A
10 ml Solution B
10 ml Solution C
10 ml Solution D
10 ml Solution E
10 ml Solution F
10 ml Solution G
2 ml Trace elements I
2 ml Trace elements II
200 ml Supplement 1
2 ml Supplement 2
2 ml Supplement 3
2 ml Supplement 5
200 ml Supplement 4

Compound	Conc. [g/L]	Conc. [mM]
NaCl	200	3422.31
MgCl₂ x 7 H₂O	40	420.122
Agar	40	118.931
K₂SO₄	5	28.693
Casamino acids	4	-
L-Glutamic acid	2	13.593
Trisodium citrate x 2 H₂O	2	6.8
Yeast extract	2	-
NH₄Cl	1	18.695
KH₂PO₄	1	7.348
FeSO₄	0.004	0.026
Na₂S₂O₃ x 5 H₂O	0.004	0.016
CaCl₂ x 2 H₂O	0.002	0.014
CoCl₂ x 6 H₂O	6.0e-4	0.005
NiCl₂ x 6 H₂O	2.0e-4	0.002
ZnCl₂	2.0e-4	0.001
MnCl₂ x 4 H₂O	2.0e-4	0.001
Na₂MoO₄ x 2 H₂O	2.0e-4	8.3e-4
AlCl3	8.0e-5	6.0e-4
Na₂WO₄ x 2 H₂O	4.0e-5	1.2e-4
H₃BO₄	4.0e-5	-
CuCl₂ x 6 H₂O	2.0e-5	1.5e-4
BaCl2 x 2 H₂O	2.0e-5	8.2e-5
Distilled water	-	-
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Solutions:

500 ml Mineral Salts Solution for solid Medium
500 ml Mineral Salts Solution for liquid Medium
1 ml Trace Element Solution
1 ml Trace Element Solution

Compound	Conc. [g/L]	Conc. [mM]
Fetal bovine serum	100	-
Tryptose-phosphate broth	50	-
D-Glucose	18.018	100.011
HEPES	5.958	25
NaHCO₃	2.5	29.759
lipoprotein concentrate	1	-
L-Glutamine	0.5	3.421
L-Proline	0.45	3.909
L-Aspartic acid	0.449	3.37
alpha-ketoglutaric acid	0.449	3.07
L-Glutamic acid	0.25	1.699
NaOH	0.2	5
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Compound	Conc. [g/L]	Conc. [mM]
D-Glucose	6.254	34.715
L-Glutamic acid	1.397	9.493
L-Proline	0.838	7.279
DL-aspartic acid	0.835	6.275
alpha-ketoglutaric acid	0.835	5.717
L-Glutamine	0.292	2
Ascorbic acid	0.279	1.586
Glutathione	0.279	0.909
Para-aminobenzoic acid	0.028	0.204
FeSO₄ x 7 H₂O	0.014	0.05
Vitamin B₁₂	0.014	0.01
D-Biotin	0.003	0.011
ZnSO₄ x 7 H₂O	5.6e-4	0.002
MnSO₄ x 1 H₂O	4.5e-4	0.003
CoCl₂ x 6 H₂O	5.6e-5	4.3e-4
Na₂SeO₃	5.6e-5	3.2e-4
Na₂ MoO₄ x 2 H₂O	5.6e-5	2.3e-4
CuSO₄ x 5 H₂O	5.6e-5	2.2e-4
Demineralized water	-	-
Tryptose-phosphate	-	-
FBS	-	-
cholesterol lipid concentrate	-	-
L-15 powder	-	-
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Solutions:

1 ml Stock Solution D (10 ml)
1 ml Vitamin Stock (10 ml)
0.1 ml Stock Solution A (10 ml)
0.1 ml Stock Solution B (10 ml)
0.1 ml Stock Solution C (10 ml)

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