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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Lipoic acid

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315 BACTEROIDES CELLULOSOLVENS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Cellulose 5 -
NaHCO3 2 23.808
NH4Cl 0.68 12.713
K2HPO4 0.3 1.722
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
KH2PO4 0.18 1.323
(NH4)2SO4 0.15 1.135
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
Resazurin 1.0e-3 0.004
FeSO4 x 7 H2O 1.0e-3 0.004
NiCl2 x 6 H2O 2.5e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Nicotinic acid 5.0e-5 4.1e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
Lipoic acid 5.0e-5 2.4e-4
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Solutions:
  • 10 ml Trace element solution
  • 10 ml Vitamin solution
Gas phase:
  • 80 % N2
  • 20 % CO2

598 HALOVIBRIO VARIABILIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 95 1625.6
MgSO4 x 7 H2O 81 328.632
Yeast extract 7.5 -
Proteose peptone 2.5 -
KCl 1 13.414
Na2-EDTA 0.005 0.015
H3BO3 0.003 0.049
CoCl2 x 6 H2O 0.002 0.015
FeSO4 x 7 H2O 0.002 0.007
ZnSO4 x 7 H2O 1.0e-3 0.003
MnCl2 x 4 H2O 3.0e-4 0.002
Na2MoO4 x 2 H2O 3.0e-4 0.001
NiCl2 x 6 H2O 2.0e-4 0.002
CuCl2 x 2 H2O 1.0e-4 5.9e-4
Pyridoxine hydrochloride 1.0e-5 4.9e-5
Nicotinic acid 5.0e-6 4.1e-5
p-Aminobenzoic acid 5.0e-6 3.6e-5
Lipoic acid 5.0e-6 2.4e-5
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Solutions:
  • 10 ml Trace element solution SL-4
  • 10 ml Vitamin solution

1121 MMJS MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
MgSO4 x 7 H2O 4 16.229
Sulfur 3 93.545
MgCl2 x 6 H2O 3 14.756
Na2S2O3 x 5 H2O 3 12.088
NaHCO3 2 23.808
NH4Cl 1.25 23.369
CaCl2 x 2 H2O 0.8 5.442
KCl 0.33 4.427
K2HPO4 0.09 0.517
KH2PO4 0.07 0.514
Nitrilotriacetic acid 0.015 0.078
Fe(III) citrate 0.01 0.041
FeSO4 x 7 H2O 0.01 0.036
Fe2(SO4)3 x n H2O 0.01 0.025
CoSO4 x 7 H2O 0.005 0.032
MnSO4 x n H2O 0.005 -
ZnSO4 x 7 H2O 0.002 0.006
NiCl2 x 6 H2O 1.0e-3 0.008
H2WO4 1.0e-3 0.004
Na2SeO3 x 5 H2O 1.0e-3 0.004
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
NaBr 1.0e-4 9.7e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
KI 1.0e-4 3.9e-4
SrCl2 x 6 H2O 1.0e-4 3.8e-4
Pyridoxine hydrochloride 1.0e-5 4.9e-5
Na2MoO4 x 2 H2O 1.0e-5 4.1e-5
Nicotinic acid 5.0e-6 4.1e-5
p-Aminobenzoic acid 5.0e-6 3.6e-5
Lipoic acid 5.0e-6 2.4e-5
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Solutions:
  • 10 ml Trace mineral solution
  • 1 ml Vitamin solution
Gas phase:
  • 80 % N2
  • 20 % CO2

1131 HYDROGENIVIRGA OKINAWENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 4.139 20.357
Sulfur 3 93.545
NaHCO3 1 11.904
NaNO3 1 11.765
NH4Cl 0.495 9.255
KCl 0.327 4.383
K2HPO4 0.139 0.796
MgSO4 x 7 H2O 0.03 0.121
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.169
MnSO4 x n H2O 0.005 -
NiCl2 x 6 H2O 9.9e-4 0.008
CaCl2 x 2 H2O 9.9e-4 0.007
CoSO4 x 7 H2O 9.9e-4 0.006
Na2SeO3 x 5 H2O 9.9e-4 0.004
FeSO4 x 7 H2O 9.9e-4 0.004
ZnSO4 x 7 H2O 9.9e-4 0.003
H3BO3 9.9e-5 0.002
Pyridoxine hydrochloride 9.9e-5 4.8e-4
Na2MoO4 x 2 H2O 9.9e-5 4.1e-4
CuSO4 x 5 H2O 9.9e-5 4.0e-4
AlK(SO4)2 9.9e-5 3.8e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
Lipoic acid 5.0e-5 2.4e-4
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Solutions:
  • 1000 ml Modified MJ synthetic seawater
  • 10 ml Vitamin solution
  • 10 ml Modified Wolin's mineral solution II
Gas phase:
  • 80 % H2
  • 20 % CO2

1149 OCEANITHERMUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
Yeast extract 2 -
NaNO3 1.7 20.001
K2HPO4 0.78 4.478
KH2PO4 0.75 5.511
NH4Cl 0.53 9.908
MgCl2 x 6 H2O 0.36 1.771
Na2SO4 0.14 0.986
Nitrilotriacetic acid 0.128 0.67
CaCl2 x 2 H2O 0.1 0.68
FeCl3 x 6 H2O 0.014 0.05
NiCl2 x 6 H2O 0.001 0.009
ZnCl2 1.0e-3 0.007
MnCl2 x 4 H2O 1.0e-3 0.005
Na2SeO3 x 5 H2O 2.6e-4 9.9e-4
CuCl2 x 2 H2O 2.5e-4 0.001
CoCl2 x 6 H2O 2.4e-4 0.002
Na2MoO4 x 2 H2O 2.4e-4 9.9e-4
H3BO3 1.0e-4 0.002
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Nicotinic acid 5.0e-5 4.1e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
Lipoic acid 5.0e-5 2.4e-4
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Solutions:
  • 10 ml Trace element solution
  • 10 ml Vitamin solution
Gas phase:
  • 80 % N2
  • 20 % CO2

1151 SDO MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 100 1711.16
Agar 20 59.465
MgSO4 x 7 H2O 3.45 13.997
MgCl2 x 6 H2O 3 14.756
NaHCO3 1 11.904
NH4Cl 0.25 4.674
CaSO4 x 2 H2O 0.25 1.452
KH2PO4 0.14 1.029
CaCl2 x 2 H2O 0.14 0.952
Yeast extract 0.1 -
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
MnCl2 x 4 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
NiCl2 x 6 H2O 2.0e-5 1.5e-4
CuCl2 x 2 H2O 1.0e-5 5.9e-5
Pyridoxine hydrochloride 1.0e-5 4.9e-5
Nicotinic acid 5.0e-6 4.1e-5
p-Aminobenzoic acid 5.0e-6 3.6e-5
Lipoic acid 5.0e-6 2.4e-5
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Solutions:
  • 1 ml Trace element solution SL-6
  • 1 ml Vitamin solution

1165 SPIROCHAETA AMERICANA MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 24 285.69
D-Glucose 5 27.753
Na2CO3 2.76 26.041
NH4Cl 1 18.695
Yeast extract 0.5 -
Na2S x 9 H2O 0.4 1.665
KCl 0.2 2.683
K2HPO4 0.2 1.148
MgCl2 x 6 H2O 0.1 0.492
MnCl2 x 4 H2O 0.004 0.018
Fe(NH4)2(SO4)2 x 12 H2O 0.002 -
CoCl2 x 6 H2O 1.0e-3 0.008
Resazurin 1.0e-3 0.004
FeSO4 x 7 H2O 1.0e-3 0.004
ZnSO4 x 7 H2O 1.0e-3 0.003
NiCl2 5.0e-4 0.004
H3BO3 1.0e-4 0.002
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
AlK(SO4)2 x 12 H2O 1.0e-4 2.1e-4
Pyridoxine hydrochloride 2.0e-5 9.7e-5
Nicotinic acid 1.0e-5 8.1e-5
p-Aminobenzoic acid 1.0e-5 7.3e-5
Lipoic acid 1.0e-5 4.8e-5
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Solutions:
  • 2 ml Vitamin solution
  • 1 ml Trace element solution

1200 ANAEROMYXOBACTER-MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.545 54.108
NaCl 1.006 17.208
MgCl2 x 6 H2O 0.503 2.473
Na-acetate 0.402 4.904
Disodium fumarate 0.364 2.272
NH4Cl 0.302 5.64
KCl 0.302 4.047
KH2PO4 0.201 1.478
DL-Dithiothreitol 0.14 0.907
CaCl2 x 2 H2O 0.015 0.103
Na2S x H2O 0.015 0.186
L-Cysteine 0.014 0.113
HCl 0.002 0.053
FeSO4 x 7 H2O 0.001 0.005
NaOH 0.001 0.025
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
Pyridoxine hydrochloride 1.8e-4 8.8e-4
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Nicotinic acid 9.1e-5 7.4e-4
p-Aminobenzoic acid 9.1e-5 6.6e-4
Lipoic acid 9.1e-5 4.4e-4
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Solutions:
  • 10 ml Solution A
  • 1 ml Solution B
  • 0.2 ml Solution C
  • 0.2 ml Solution D ("red mix")
  • 0.05 ml Solution E (electron acceptor)
  • 0.2 ml Solution F: Vitamin solution according to Wolin and coauthors
  • 1 ml Trace element solution SL-10 B
  • 2 ml Selenite-tungstate solution

1275 GEORGFUCHSIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.391 40.365
NaNO3 0.773 9.091
KH2PO4 0.653 4.798
NH4HCO3 0.4 -
Na2HPO4 0.201 1.415
CaCl2 0.1 0.901
MgCl2 0.091 0.955
L-Cysteine HCl x H2O 0.027 0.155
HCl 0.003 0.069
NaOH 0.002 0.05
FeCl2 x 4 H2O 0.002 0.008
Resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Pyridoxine hydrochloride 9.1e-5 4.4e-4
ZnCl2 7.0e-5 5.2e-4
Nicotinic acid 4.5e-5 3.7e-4
p-Aminobenzoic acid 4.5e-5 3.3e-4
Lipoic acid 4.5e-5 2.2e-4
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Solutions:
  • 10 ml Solution A
  • 0.5 ml Solution B
  • 0.5 ml Solution C
  • 0.25 ml Solution D
  • 0.4 ml Solution E
  • 1 ml Trace element solution SL-10
  • 4 ml Selenite-tungstate solution
  • 1 ml Vitamin solution

1282 SH SEAWATER MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 24.32 416.153
Agar 15 44.599
MgCl2 x 6 H2O 10 49.188
Na2SO4 4 28.161
HEPES 2.38 9.987
CaCl2 x 2 H2O 1.5 10.203
KCl 0.66 8.853
NaHCO3 0.2 2.381
Peptone 0.2 -
Yeast extract 0.1 -
KBr 0.1 0.84
H3BO3 0.025 0.4
SrCl2 0.024 0.15
NH4Cl 0.021 0.4
Na2MoO4 x 2 H2O 0.01 0.041
KH2PO4 0.005 0.04
NH4NO3 0.003 -
NaF 0.003 0.07
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 1.0e-4 -
ZnCl2 3.6e-5 2.6e-4
Nicotinic acid 2.5e-5 2.0e-4
Para-aminobenzoic acid 2.5e-5 1.8e-4
Pyridoxine hydrochloride 2.5e-5 1.2e-4
Lipoic acid 2.5e-5 1.2e-4
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Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamin solution
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