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Search for media using compounds and concentrations.

The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Methanol

Your search received 69 results.

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

120 METHANOSARCINA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
Na-acetate 2.441 29.761
NaCl 2.197 37.599
NaHCO3 1.953 23.25
Casitone 1.953 -
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 100 % N2
  • 20 % CO2

120a METHANOSARCINA BARKERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
NaCl 2.197 37.599
Yeast extract 1.953 -
Casitone 1.953 -
NaHCO3 0.83 9.881
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
  • 20 % CO2

120b METHANOMICROCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
Na-acetate 2.441 29.761
NaHCO3 2.441 29.062
NaCl 2.197 37.599
Yeast extract 1.953 -
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

120d METHANOSARCINA MARINE MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 22.461 384.342
Methanol 15.469 482.765
MgCl2 x 6 H2O 9.766 48.035
Na2CO3 0.977 9.214
NH4Cl 0.488 9.128
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
  • 20 % CO2

233 METHANOLOBUS TINDARIUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.742 241.618
MgCl2 x 6 H2O 3.91 19.233
NaHCO3 0.978 11.636
L-Cysteine HCl x H2O 0.489 2.783
Na2S x 9 H2O 0.489 2.035
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

242 BMM - MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
K2HPO4 7 40.189
(NH4)2SO4 3 22.703
NaCl 2 34.223
KH2PO4 2 14.697
MgSO4 x 7 H2O 0.5 2.029
Yeast extract 0.1 -
FeSO4 x 7 H2O 0.01 0.036
MnSO4 x H2O 0.006 0.037
Thiamine-HCl x 2 H2O 1.0e-4 2.7e-4
Biotin 1.0e-5 4.1e-5
Methanol - -
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317 METHANOSARCINA THERMOPHILA MEDIUM

Compound Conc. [g/L] Conc. [mM]
NH4Cl 1 18.695
Na2CO3 1 9.435
Yeast extract 1 -
L-Cysteine HCl x H2O 0.5 2.847
K2HPO4 0.4 2.297
(NH4)2SO4 0.3 2.27
KH2PO4 0.3 2.205
Na2S x 9 H2O 0.25 1.041
MgCl2 x 6 H2O 0.2 0.984
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
Sodium resazurin 5.0e-4 -
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
Distilled water - -
Methanol - -
Solutions:
  • 50 ml Minerals solution
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % N2
  • 20 % CO2

318 METHANOSARCINA THERMOPHILA (BCYT) MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.757 242.092
KHCO3 1.959 19.566
NH4Cl 0.979 18.311
Yeast extract 0.49 -
Trypticase peptone 0.49 -
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Solutions:
  • 10 ml Trace element solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

322 METHANOSPHAERA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.704 240.443
NaHCO3 3.891 46.318
KH2PO4 2.724 20.015
Trypticase peptone 1.946 -
Yeast extract 1.946 -
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Solutions:
  • 100 ml Clarified rumen fluid
  • 10 ml Modified Wolin's mineral solution
  • 2 ml Wolin's vitamin solution (10x)
  • 3 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2
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